Department of Chemistry, Middle East Technical University, 06800, Çankaya, Ankara, Turkey.
Anal Bioanal Chem. 2022 Oct;414(24):7299-7313. doi: 10.1007/s00216-022-04282-2. Epub 2022 Aug 17.
In this study, a novel, rapid, and sensitive fluorescence sensing platform was developed for the detection of coralyne (COR) by the conjugation of coumarin 6H (C6H) fluorescent dye with oligonucleotide-modified silver nanoparticles [(dT)-AgNPs]. In the presence of COR, a remarkable and rapid decrease in the fluorescence signal of the probe with a quenching efficiency of around 62% was observed. The quenching response of the system towards COR was possibly due to the displacement of thymidine-rich deoxyoligonucleotides by COR on the surface of AgNPs. The complementary experiments with an adenine-rich single strand as well as with two different secondary structures (i.e., duplex and triplex) revealed a favorable sequence specificity of the sensing platform. The influence of key parameters including the incubation time and temperature was evaluated and optimized to achieve the highest performance. The linear range of 10-183 nM with a correlation coefficient of R = 0.9982 and a limit of detection of 5.24 nM were obtained under the optimized conditions. The selectivity of the proposed probe towards COR was revealed by the evaluation of its response to other small molecules that have molecular structures similar to COR. Finally, the successful applicability of the system was shown with the obtained average recoveries in the range of 87.28-104.52% in human urine samples.
在这项研究中，通过将香豆素 6H（C6H）荧光染料与寡核苷酸修饰的银纳米粒子[(dT)-AgNPs]缀合，开发了一种用于检测珊瑚宁（COR）的新型、快速和灵敏的荧光传感平台。在 COR 的存在下，观察到探针的荧光信号显著且快速下降，猝灭效率约为 62%。该系统对 COR 的猝灭响应可能归因于 COR 在 AgNPs 表面取代富含胸腺嘧啶的脱氧寡核苷酸。用富含腺嘌呤的单链以及两种不同的二级结构（即双链和三链）进行的互补实验揭示了传感平台的有利序列特异性。评估并优化了包括孵育时间和温度在内的关键参数，以获得最佳性能。在优化条件下，获得了 10-183 nM 的线性范围，相关系数 R=0.9982，检测限为 5.24 nM。通过评估其对具有与 COR 相似分子结构的其他小分子的响应，揭示了该探针对 COR 的选择性。最后，通过在人尿样中获得 87.28-104.52%的平均回收率，展示了该系统的成功适用性。
