Department of Physics, National Dong Hwa University, Shoufeng, Hualien, Taiwan 974.
Department of Chemistry, National Dong Hwa University, Shoufeng, Hualien, Taiwan 974.
Anal Chem. 2022 Aug 30;94(34):11734-11738. doi: 10.1021/acs.analchem.2c02807. Epub 2022 Aug 17.
Polyhydroxyalkanoate (PHA) is one of the biocompatible and biodegradable plastics that can be produced and accumulated as granules inside microorganisms. In this study, a new approach to rapidly quantify a short-chain-length PHA, polyhydroxybutyrate (PHB), produced from genetically engineered containing is presented. The mass of each bacterial cell was measured using a laser-induced radio frequency (rf) plasma charge detection quadrupole ion trap mass spectrometer (LIRFP CD QIT-MS), and then, the PHB contents were determined by calculating the change in cellular mass. The quantitative results showed that the PHB contents measured by LIRFP CD QIT-MS were consistent with those by reference analysis, gas chromatography (GC). The PHB content of each bacterial sample can be obtained within 20 min from sampling using LIRFP CD QIT-MS while GC analysis takes 2 days. In addition, LIRFP CD QIT-MS does not use any hazardous chemicals in cellular mass quantification as compared to GC. This indicates that LIRFP CD QIT-MS has potential in routine monitoring of PHB production.
聚羟基烷酸酯（PHA）是一种生物相容性和可生物降解的塑料，可以作为颗粒在微生物体内产生和积累。在这项研究中，提出了一种快速定量分析由基因工程 产生的短链长聚羟基丁酸酯（PHB）的新方法。使用激光诱导射频（rf）等离子体电荷检测四极离子阱质谱仪（LIRFP CD QIT-MS）测量每个细菌细胞的质量，然后通过计算细胞质量的变化来确定 PHB 的含量。定量结果表明，LIRFP CD QIT-MS 测量的 PHB 含量与参考分析（气相色谱法（GC））一致。使用 LIRFP CD QIT-MS 从采样到获得每个细菌样品的 PHB 含量，仅需 20 分钟，而 GC 分析则需要 2 天。此外，与 GC 相比，LIRFP CD QIT-MS 在细胞质量定量分析中不使用任何危险化学品。这表明 LIRFP CD QIT-MS 在 PHB 生产的常规监测中有潜力。
