Department of Medical Laboratory Sciences, School of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Biochemistry, School of Medicine, AJA University of Medical Sciences, Tehran, Iran.
BMC Endocr Disord. 2022 Aug 17;22(1):207. doi: 10.1186/s12902-022-01120-5.
In view of the growing global prevalence of type 2 diabetes (T2D), detection of prediabetes and type 2 diabetes in the early stages is necessary to reduce the risk of developing diabetes, prevent the progression of the disease, and dysfunction of different organs. Since miRNAs are involved in the initiation and progression of numerous pathogenic processes, including diabetes, in the present study, we aimed to investigate the expression of miR-148b-3p and miR-27a-3p in prediabetic and T2D patients and to evaluate the diagnostic potential of these miRNAs.
We evaluated the expression of miR-148b-3p and miR-27a-3p in the plasma of three groups: 20 prediabetic patients, 20 T2D patients, and 20 healthy controls. The biochemical parameters were determined by the auto-analyzer. The possible target genes of these miRNAs were identified using an in-silico approach.
Our results showed that, as compared to the healthy controls, there was a significant up regulation and down regulation in the expression of miR-148b-3p and miR-27a-3p in the T2D patients, respectively. The results of receiver operating characteristic curve analysis also suggested that miR-148b-3p acted successfully in discriminating the prediabetic and diabetic patients from the control group. According to in-silico analysis, miRs influence biological pathways involved in T2DM development, such as insulin signaling.
The miR148b-3p and miR-27a-3p expression levels were deregulated in diabetes and pre-diabetes. Furthermore, miR-148b-3p showed significant ability in discriminating between diabetic and healthy individuals, suggesting a potential diagnostic use of miR-148b-3p in the detection of T2D.
鉴于 2 型糖尿病（T2D）在全球的患病率不断上升，早期发现糖尿病前期和 2 型糖尿病对于降低糖尿病发病风险、防止疾病进展和不同器官功能障碍非常必要。由于 miRNAs 参与了包括糖尿病在内的许多致病过程的启动和进展，因此在本研究中，我们旨在研究 miR-148b-3p 和 miR-27a-3p 在糖尿病前期和 T2D 患者中的表达，并评估这些 miRNAs 的诊断潜力。
我们评估了三组血浆中 miR-148b-3p 和 miR-27a-3p 的表达水平：20 例糖尿病前期患者、20 例 T2D 患者和 20 例健康对照者。通过自动分析仪测定生化参数。使用计算机模拟方法鉴定这些 miRNA 的可能靶基因。
与健康对照组相比，T2D 患者的 miR-148b-3p 和 miR-27a-3p 表达均显著上调和下调。ROC 曲线分析的结果也表明，miR-148b-3p 可成功区分糖尿病前期和糖尿病患者与对照组。根据计算机模拟分析，miRs 影响了 T2DM 发展涉及的生物途径，如胰岛素信号转导。
miR148b-3p 和 miR-27a-3p 的表达水平在糖尿病和糖尿病前期失调。此外，miR-148b-3p 在区分糖尿病患者和健康个体方面表现出显著的能力，提示 miR-148b-3p 在检测 T2D 方面具有潜在的诊断用途。
