Clinical Biochemistry Department, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran.
Clinical Biochemistry Department, Faculty of Medicine, Tarbiat Modares University, Tehran, Iran.
BMC Pharmacol Toxicol. 2022 Aug 17;23(1):63. doi: 10.1186/s40360-022-00604-3.
High glucose conditions cause some changes in the vessels of diabetes through the signal transduction pathways. Dexamethasone and other corticosteroids have a wide range of biological effects in immunological events. In the present study, the effects of dexamethasone were investigated on the VSMC (vascular smooth muscle cell) proliferation, and migration based on the FAK gene and protein changes in high glucose conditions.
The vascular smooth muscle cells were cultured in DMEM and were treated with dexamethasone (10 M, 10 M, and 10 M) for 24, and 48 h in high glucose conditions. The cell viability was estimated by MTT method. The FAK gene expression levels and pFAK protein values were determined by RT-qPCR and western blotting techniques, respectively. A scratch assay was used to evaluate cellular migration.
The FAK gene expression levels decreased significantly dependent on dexamethasone doses at 24 and 48 h. The pFAK protein values decreased significantly with a time lag at 24- and 48-h periods as compared with gene expression levels.
The results showed that the inhibition of VSMC proliferation and migration by dexamethasone in the high glucose conditions may be related to the changes of FAK.
高糖条件通过信号转导通路引起糖尿病血管的某些变化。地塞米松和其他皮质类固醇在免疫事件中具有广泛的生物学效应。在本研究中，基于高糖条件下 FAK 基因和蛋白的变化，研究了地塞米松对血管平滑肌细胞（VSMC）增殖和迁移的影响。
将血管平滑肌细胞在 DMEM 中培养，并在高糖条件下用地塞米松（10μM、10μM 和 10μM）处理 24 和 48 小时。通过 MTT 法估计细胞活力。通过 RT-qPCR 和 Western blot 技术分别测定 FAK 基因表达水平和 pFAK 蛋白值。划痕实验用于评估细胞迁移。
FAK 基因表达水平在 24 和 48 小时内随地塞米松剂量的增加而显著降低。pFAK 蛋白值在 24-48 小时内与基因表达水平呈时间滞后性显著降低。
结果表明，地塞米松在高糖条件下抑制 VSMC 增殖和迁移可能与 FAK 的变化有关。
