Department of Pharmacy, Medical Supplies Center of PLA General Hospital, the Second Military Medical University of PLA, Beijing 100853, China.
Department of Pharmacy, Medical Supplies Center of PLA General Hospital, Shenyang Pharmaceutical University, Beijing 100853, China.
Biomed Res Int. 2022 Aug 8;2022:1636301. doi: 10.1155/2022/1636301. eCollection 2022.
The incidence of diabetes is increasing year by year; among them, the rising trend of T2D is particularly obvious, and because it has many complications and poor prognosis, it has become one of the diseases that seriously endanger human health. Gegen Qinlian Decoction (GQD) has achieved good results in the treatment of type 2 diabetes (T2D). It can not only improve the anti-insulin effect of nude mice with type 2 diabetic, but also has the characteristics of small side effects and remarkable curative effect; it can improve the function of tissues and organs by multiple target spots and multiple ways. The method was to raise 30 nude mice with high fat and high sugar and inject small doses of streptozotocin (STZ). The treated nude mice were randomly divided into rosiglitazone group, Gegen Qinlian group, and model group, normally rearing 10 nude mice as a control group. The model group and the blank group were treated with 0.9% sodium chloride solution at 10 mL/kg, and the rosiglitazone group was intragastric administration with 3 mg/kg rosiglitazone solution. The GQD group was treated with 18.2 g/kg GQD, once a day, and lasting for 4 weeks. Use blood glucose meter to detect the FBG content in nude mice, use the radioimmunoassay to detect the amount of FINS, and calculate HOMA-IR. Use immunoenzyme linked assay method to detect the level of serum inflammatory factors. The experimental results showed that compared with the model group, the FBG of the rosiglitazone group mice was significantly decreased; the levels of the rosiglitazone group and the Gegen Qinlian group were significantly lower. Therefore, it is concluded that GQD can treat T2D in nude mice.
糖尿病的发病率逐年上升；其中，2 型糖尿病（T2D）的上升趋势尤为明显，由于其并发症多、预后差，已成为严重危害人类健康的疾病之一。葛根芩连汤（GQD）在治疗 2 型糖尿病（T2D）方面取得了良好的效果。它不仅可以改善 2 型糖尿病裸鼠的抗胰岛素作用，而且具有副作用小、疗效显著的特点；它可以通过多个靶点和多种途径改善组织和器官的功能。方法是饲养 30 只高脂高糖的裸鼠，并注射小剂量链脲佐菌素（STZ）。将处理后的裸鼠随机分为罗格列酮组、葛根芩连组和模型组，正常饲养 10 只裸鼠作为对照组。模型组和空白组用 0.9%氯化钠溶液 10 ml/kg 处理，罗格列酮组用 3mg/kg 罗格列酮溶液灌胃。GQD 组用 18.2 g/kg GQD 治疗，每天一次，持续 4 周。用血糖仪检测裸鼠的 FBG 含量，用放射免疫法检测 FINS 量，并计算 HOMA-IR。用酶联免疫吸附法检测血清炎症因子水平。实验结果表明，与模型组相比，罗格列酮组小鼠的 FBG 明显降低；罗格列酮组和葛根芩连组的水平明显降低。因此，结论是 GQD 可以治疗 T2D 裸鼠。
