Australian Research Council Centre of Excellence for Innovations in Peptide and Protein Science, Edith Cowan University, School of Science, 270 Joondalup Dr, Joondalup, WA 6027, Australia.
CSIRO Agriculture and Food, 306 Carmody Rd, St. Lucia, QLD 4067, Australia.
J Agric Food Chem. 2022 Aug 31;70(34):10680-10691. doi: 10.1021/acs.jafc.2c03816. Epub 2022 Aug 18.
Barley is one of the key cereal grains for malting and brewing industries. However, climate variability and unprecedented weather events can impact barley yield and end-product quality. The genetic background and environmental conditions are key factors in defining the barley proteome content and malting characteristics. Here, we measure the barley proteome and malting characteristics of three barley lines grown in Western Australia, differing in genetic background and growing location, by applying liquid chromatography-mass spectrometry (LC-MS). Using data-dependent acquisition LC-MS, 1571 proteins were detected with high confidence. Quantitative data acquired using sequential window acquisition of all theoretical (SWATH) MS on barley samples resulted in quantitation of 920 proteins. Multivariate analyses revealed that the barley lines' genetics and their growing locations are strongly correlated between proteins and desired traits such as the malt yield. Linking meteorological data with proteomic measurements revealed how high-temperature stress in northern regions affects seed temperature tolerance during malting, resulting in a higher malt yield. Our results show the impact of environmental conditions on the barley proteome and malt characteristics; these findings have the potential to expedite breeding programs and malt quality prediction.
大麦是麦芽和酿造行业的主要谷物之一。然而，气候变异性和前所未有的天气事件会影响大麦的产量和最终产品质量。遗传背景和环境条件是定义大麦蛋白质组含量和制麦特性的关键因素。在这里，我们通过液相色谱-质谱（LC-MS）测量了在西澳大利亚种植的三个大麦品种的大麦蛋白质组和制麦特性，这些品种在遗传背景和种植地点上存在差异。使用基于数据依赖性采集的 LC-MS，我们检测到了 1571 种具有高可信度的蛋白质。对大麦样品进行顺序窗口采集所有理论（SWATH）MS 定量数据后，定量了 920 种蛋白质。多变量分析表明，大麦品种的遗传背景和生长地点与其生长地点之间存在很强的相关性，与所需特性（如麦芽产量）相关的蛋白质也存在很强的相关性。将气象数据与蛋白质组学测量结果联系起来，揭示了北部地区高温胁迫如何影响制麦过程中的种子温度耐受性，从而导致更高的麦芽产量。我们的研究结果表明了环境条件对大麦蛋白质组和麦芽特性的影响；这些发现有可能加速育种计划和麦芽质量预测。
