Fu Zi, Qu Wen, Shao Zong-Hong, Wang Hua-Quan, Xing Li-Min, Dong Xi-Feng, Liu Zhao-Yun, Li Xiao-Na, Zhang Yang, Ding Shao-Xue
Department of Hematology, Tianjin Medical University General Hospital, Tianjin 300052, China.
Department of Hematology, Tianjin Medical University General Hospital, Tianjin 300052, China.E-mail:
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2022 Aug;30(4):1170-1175. doi: 10.19746/j.cnki.issn.1009-2137.2022.04.030.
To investigate the expression of programmed death receptor-1 (PD-1) and inducible costimulator (ICOS) on the surface of CD8 T cells in peripheral blood of patients with primary immune thrombocytopenia (ITP), and explore the roles of PD-1 and ICOS in the occurrence and development of ITP.
A total of 28 ITP patients treated in Tianjin Medical University General Hospital from September to December 2020 were selected, including 13 patients with newly diagnosed ITP, 15 patients with chronic ITP, and 22 healthy volunteers were recruited as control group. Flow cytometry was used to detect the expression levels of PD-1 and ICOS, and evaluate their correlation with clinical indicators.
The percentage of CD8 T cells in ITP patients of chronic group was higher than that of the newly diagnosed group and the control group (P<0.05). The expression level of PD-1 on CD8 T cells in ITP patients of newly diagnosed group and chronic group were significantly lower than that of the control group (P<0.05), while the expression level of ICOS were significantly higher (P<0.05). In ITP patients, PD-1 was negatively correlated with platelet count (r=-0.4942, P<0.01), but positively with ICOS (r=0.4342). PD-1 and ICOS were both negatively correlated with lymphocyte count (r=-0.4374; r=-0.4492).
In ITP patients, the unbalanced expression of PD-1 and ICOS may interfere with the immune homeostasis of the body, which can be used as a therapeutic target for ITP patients.
探讨原发性免疫性血小板减少症（ITP）患者外周血CD8⁺ T细胞表面程序性死亡受体-1（PD-1）和诱导性共刺激分子（ICOS）的表达情况，探讨PD-1和ICOS在ITP发生发展中的作用。
选取2020年9月至12月在天津医科大学总医院治疗的28例ITP患者，其中新诊断ITP患者13例，慢性ITP患者15例，并招募22名健康志愿者作为对照组。采用流式细胞术检测PD-1和ICOS的表达水平，并评估其与临床指标的相关性。
慢性组ITP患者CD8⁺ T细胞百分比高于新诊断组和对照组（P<0.05）。新诊断组和慢性组ITP患者CD8⁺ T细胞表面PD-1表达水平均显著低于对照组（P<0.05），而ICOS表达水平显著高于对照组（P<0.05）。在ITP患者中，PD-1与血小板计数呈负相关（r=-0.4942，P<0.01），但与ICOS呈正相关（r=0.4342）。PD-1和ICOS均与淋巴细胞计数呈负相关（r=-0.4374；r=-0.4492）。
ITP患者中PD-1和ICOS的表达失衡可能干扰机体免疫稳态，可作为ITP患者的治疗靶点。
