Song Jie, Chen Li Ying-Hui, Liu Ai-Ping, Wang Shu-Ya, Lyu Xian-Ping
Department of Blood Transfusion, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan Province, China.
Department of Blood Transfusion, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan Province, China .E-mail:
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2022 Aug;30(4):1198-1202. doi: 10.19746/j.cnki.issn.1009-2137.2022.04.035.
To investigate the effectiveness and safety of low concentration dithiothreitol (DTT) in removing the interference of monoclonal anti-CD38 on transfusion compatibility testing, and develop a reasonable clinical transfusion strategy.
The blood type, direct antiglobulin testing (DAT) and antibody screening were tested according to standard methods. Antibody screening cells and donor's red blood cells were treated by DTT 0.2, 0.1, 0.05, 0.02, 0.01 and 0.005 mol/L, and antibody screening and cross-matching of serums after monoclonal anti-CD38 treatment were performed by anti-human globulin card.
The 0.01 mol/L DTT at 37℃ for 30 minutes could remove the effect of monoclonal anti-CD38 on antibody screening and cross-matching, meanwhile retain their effectiveness in detecting anti-K, anti-LW, anti-JMH, anti-Lu, anti-e, anti-Di and anti-Jk alloantibodies. All the 10 patients had no acute or delayed haemolytic transfusion reactions and their routine blood tests showed that the red blood cells transfusion was effective.
The 0.01 mol/L DTT is a safe and effective method for removing the interference of monoclonal anti-CD38 with transfusion compatibility testing, while retaining the ability to detect most alloantibodies.
探讨低浓度二硫苏糖醇（DTT）去除单克隆抗CD38对输血相容性检测干扰的有效性及安全性，制定合理的临床输血策略。
按照标准方法进行血型、直接抗球蛋白试验（DAT）及抗体筛查。用0.2、0.1、0.05、0.02、0.01和0.005 mol/L的DTT处理抗体筛查细胞及供者红细胞，采用抗人球蛋白卡对单克隆抗CD38处理后的血清进行抗体筛查及交叉配血。
0.01 mol/L DTT在37℃孵育30分钟可消除单克隆抗CD38对抗体筛查及交叉配血的影响，同时保留其检测抗-K、抗-LW、抗-JMH、抗-Lu、抗-e、抗-Di和抗-Jk同种抗体的效能。10例患者均未发生急性或迟发性溶血性输血反应，血常规检查显示红细胞输注有效。
0.01 mol/L DTT是一种安全有效的方法，可去除单克隆抗CD38对输血相容性检测的干扰，同时保留检测大多数同种抗体的能力。
