Ding Qin-Li, Liu Ai-Fei, Qiu Fang
Department of Blood Transfusion, First Affiliated Hospital of Gannan Medical University, Ganzhou 341000, Jiangxi Province, China.
Department of Blood Transfusion, First Affiliated Hospital of Gannan Medical University, Ganzhou 341000, Jiangxi Province, China. E-mail:
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2022 Aug;30(4):1208-1212. doi: 10.19746/j.cnki.issn.1009-2137.2022.04.037.
To identify the ABO positive and negative stereotypic inconsistencies in a dizygotic twin with positive stereotypic patterns and mixed agglutination, and explore the application of serological characteristics and gene sequence in congenital blood group chimeras.
ABO blood group identification, Rh and MN typing were performed using the microcolumn gel method and ABO genotyping was performed using the PCR-SSP method.
In this patient, both anti-A and anti-B tubes had mixed hemagglutination of red blood cells, and the anti-ABO tube was AB type. The Rh typing of the patient was CcDEe. Mixed agglutination of red blood cells was observed in both anti-M and anti-N tubes in MN typing. The patient's father and mother was normal Type O and AB, respectively. There were three alleles in the ABO gene of the patient, O0101 came from his father, while A102 and B01 came from his mother.
The patient has two groups of red blood cells (type A and B). Because the patients is a dizygotic twin, these two groups of red blood cells can be chimeras formed by blood exchange between the twins. Through gene sequencing, it can be determined that the patient is a congenital A/B blood type chimera.
鉴定一对具有定型模式阳性和混合凝集的双卵双胞胎中的ABO阳性和阴性定型不一致情况，并探讨血清学特征和基因序列在先天性血型嵌合体中的应用。
采用微柱凝胶法进行ABO血型鉴定、Rh和MN定型，采用PCR-SSP法进行ABO基因分型。
该患者抗A管和抗B管均出现红细胞混合凝集，抗ABO管为AB型。患者Rh定型为CcDEe。MN定型中抗M管和抗N管均观察到红细胞混合凝集。患者的父亲和母亲血型正常，分别为O型和AB型。患者ABO基因中有三个等位基因，O0101来自其父亲，而A102和B01来自其母亲。
该患者有两组红细胞（A型和B型）。由于患者是双卵双胞胎，这两组红细胞可能是双胞胎之间血液交换形成的嵌合体。通过基因测序，可以确定该患者为先天性A/B血型嵌合体。
