Katzman M, Lederman M M, Spagnuolo P J
J Lab Clin Med. 1987 Jul;110(1):75-82.
We investigated a possible role for fibronectin (Fn) in natural killer (NK) cell activity against K562 tumor cells. Plastic plates that had been incubated with Fn at 60 micrograms/ml bound 49.6% +/- 12.8% (mean +/- SEM) of K562 cells; plates coated with three other proteins bound essentially no tumor cells (P less than 0.01). In similar experiments, 12.6% +/- 4.7% of nonadherent lymphocytes bound to plates that had been coated with 60 micrograms/ml Fn; plates coated with other proteins bound less than or equal to 10% of these cells. Lymphocytes that bound to Fn-coated plates appeared to be slightly enriched for NK cells as assessed by morphology and by cytotoxic activity. Despite these findings, neither NK cytotoxic activity nor binding to K562 targets was affected by the addition of any of three anti-Fn antibody preparations, nor by the addition of exogenous Fn at concentrations found in plasma (300 micrograms/ml). Although Fn appears to bind both to K562 targets and to lymphocyte preparations that contain NK cells, it does not appear to have a functional role in mediating NK cell activity against K562 tumor cells.
我们研究了纤连蛋白（Fn）在自然杀伤（NK）细胞对K562肿瘤细胞的活性中可能发挥的作用。用60微克/毫升的Fn孵育过的塑料平板结合了49.6%±12.8%（平均值±标准误）的K562细胞；涂有其他三种蛋白质的平板基本不结合肿瘤细胞（P<0.01）。在类似实验中，12.6%±4.7%的非贴壁淋巴细胞与涂有60微克/毫升Fn的平板结合；涂有其他蛋白质的平板结合这些细胞的比例小于或等于10%。通过形态学和细胞毒性活性评估，与Fn包被平板结合的淋巴细胞似乎NK细胞略有富集。尽管有这些发现，但添加三种抗Fn抗体制剂中的任何一种，以及添加血浆中浓度的外源性Fn（300微克/毫升），均不影响NK细胞毒性活性或与K562靶标的结合。虽然Fn似乎既能结合K562靶标，也能结合含有NK细胞的淋巴细胞制剂，但它在介导NK细胞对K562肿瘤细胞的活性中似乎没有功能作用。
