Department of Agricultural Biotechnology, Faculty of Agriculture, Jahrom University, Jahrom, Iran.
Department of Nephrology, Faculty of Internal Medicine, Shiraz University of Medical Science, Shiraz, Iran.
Microrna. 2022;11(3):245-262. doi: 10.2174/2211536611666220818150634.
In 2019, severe acute respiratory coronavirus II (or SARS-COV-2) emerged in Wuhan, China, rapidly becoming a global pandemic. Coronavirus genus (Coronaviridae) has the largest single-stranded positive-sense RNA genome (~30 kb) among the human infected single-stranded RNA viruses.
For the study of active therapeutic plant-derived miRNA(s), it may be possible to uptake the miRNAs and their biological role in the host cell. In this study, we bioinformatically searched plant miRNAs that can potentially interact with the Sars-CoV-2 genome within the 3'- UTR region and have prompt antiviral activity.
We searched the plant miRNAs that target the 3'-UTR flanking region of the Sars-CoV-2 genome by employing the RNAHybrid, RNA22, and STarMir miRNA/target prediction tools.
The RNAHybrid algorithm found 63 plant miRNAs having hybridization energy with less or equal to -25 kcal.mol-1. Besides, RNA22 and STarMir tools identified eight interactions between the plant miRNAs and the targeted RNA sequence. pvu-miR159a. 2 and sbi-miR5387b were predicted as the most effectively interacting miRNAs in targeting the 3'-UTR sequence, not only by the RNA22 tool but also by the STarMir tool at the same position. However, the GC content of the pvumiR159a. 2 is 55% instead of sbi-miR5387b, which is a GC enriched sequence (71.43%) that may activate TLR receptors.
In our opinion, they are potent plant-derived miRNA candidates that have a great chance of targeting the Sars-CoV-2 genome in the 3'-UTR region in vitro. Therefore, we propose pvu-miR159a.2 for studying antiviral miRNA-based therapies without any essential side effects in vivo.
2019 年，严重急性呼吸综合征冠状病毒 2 型（或 SARS-CoV-2）在中国武汉出现，迅速成为全球性大流行。冠状病毒属（ Coronaviridae ）是感染人类单链 RNA 病毒中最大的单链正链 RNA 基因组（~30kb ）。
对于研究具有活性的治疗性植物源性 miRNA ，有可能摄取 miRNA 及其在宿主细胞中的生物学作用。在这项研究中，我们通过生物信息学搜索了可以潜在与 3'-UTR 区域内的 SARS-CoV-2 基因组相互作用并具有即时抗病毒活性的植物 miRNA 。
我们使用 RNAHybrid 、 RNA22 和 STarMir miRNA /靶标预测工具，搜索靶向 SARS-CoV-2 基因组 3'-UTR 侧翼区的植物 miRNA 。
RNAHybrid 算法发现 63 种植物 miRNA 与杂交能小于或等于-25 kcal.mol-1 。此外，RNA22 和 STarMir 工具鉴定了植物 miRNA 与靶向 RNA 序列之间的 8 个相互作用。pvumiR159a.2 和 sbi-miR5387b 被预测为靶向 3'-UTR 序列的最有效相互作用 miRNA ，不仅通过 RNA22 工具，而且通过 STarMir 工具在同一位置预测。然而，pvumiR159a.2 的 GC 含量为 55%，而不是 sbi-miR5387b ，这是一个富含 GC 的序列（71.43% ），可能激活 TLR 受体。
在我们看来，它们是具有潜力的植物源性 miRNA 候选物，很有可能在体外靶向 SARS-CoV-2 基因组的 3'-UTR 区域。因此，我们建议使用 pvumiR159a.2 来研究抗病毒 miRNA 为基础的治疗方法，而不会在体内产生任何必要的副作用。
