血清 N-糖链在评估 1 型糖尿病成人早期血管老化（动脉僵硬度）中的应用。

Measurement of Serum N-Glycans in the Assessment of Early Vascular Aging (Arterial Stiffness) in Adults With Type 1 Diabetes.

机构信息

Department of Endocrinology and Nutrition, Hospital del Mar, Institut Hospital del Mar d'Investigacions Mèdiques (IMIM), Barcelona, Spain.

CIBERDEM, Instituto de Salud Carlos III, Madrid, Spain.

出版信息

Diabetes Care. 2022 Oct 1;45(10):2430-2438. doi: 10.2337/dc22-0331.

DOI:10.2337/dc22-0331

PMID:35984043

Abstract

OBJECTIVE

Vascular aging (arterial stiffness [AS]) is an inflammation-linked process that predicts macro- and microvascular complications in adults with type 1 diabetes (T1D). We evaluated the utility of measuring the inflammation-linked N-glycans GlycA and GlycB to assess vascular aging in adults with T1D.

RESEARCH DESIGN AND METHODS

Eighty-four adults with T1D (>10-year duration without cardiovascular events) and 68 healthy control subjects were evaluated for clinical characteristics (including microvascular complications in patients with T1D), aortic pulse wave velocity (aPWV) (surrogate measure of AS), and serum GlycA and GlycB (peak area [concentration] and height/width [H/W] ratio) using 1H-nuclear magnetic resonance spectroscopy.

RESULTS

Patients with T1D had higher median (interquartile range) values than healthy control subjects for (P < 0.001 for all comparisons) aPWV 7.9 (6.9-9.1) vs. 6.1 (5.5-6.7) m/s, GlycA 850.4 (781.3-916.1) vs. 652.4 (581.5-727.1) μmoL; GlycB 386.1 (353.2-426.3) vs. 310.0 (280.5-331.9) μmol/L), H/W ratio of GlycA 16.5 (14.9-18.1) vs. 15.0 (13.7-16.7), and H/W ratio of GlycB 5.0 (4.6-5.5) vs. 4.0 (3.4-4.3). Moreover, aPWV correlated (P < 0.001 for all correlations) with GlycA (r = 0.550) and GlycB (r = 0.423) concentrations and with H/W ratios of GlycA (r = 0.453) and GlycB (r = 0.510). Adjusting for potential confounders, GlycA concentration (β = 0.212, P < 0.001) and the H/W ratios of GlycA (β = 0.150, P = 0.009) and GlycB (β = 0.155, P = 0.011) remained independently associated with aPWV. C-statistics for detecting individuals with aPWV >10 m/s were 0.866 (95% CI 0.794-0.937) for GlycA levels and 0.862 (0.780-0.943) for H/W ratio of GlycB.

CONCLUSIONS

Measurement of serum GlycA and GlycB may have utility in assessing vascular aging in adults with T1D of >10-year duration and no previous cardiovascular events.

摘要

目的

血管老化（动脉僵硬度[AS]）是一个与炎症相关的过程，可预测 1 型糖尿病（T1D）成人的大血管和微血管并发症。我们评估了测量与炎症相关的 N-糖链 GlycA 和 GlycB 来评估 T1D 成人的血管老化的效用。

研究设计和方法

评估了 84 名 T1D 成人（无心血管事件超过 10 年）和 68 名健康对照者的临床特征（包括 T1D 患者的微血管并发症）、主动脉脉搏波速度（aPWV）（AS 的替代指标）和血清 GlycA 和 GlycB（峰面积[浓度]和高度/宽度[H/W]比），使用 1H-核磁共振光谱法。

结果

与健康对照组相比，T1D 患者的中位数（四分位距）值更高（所有比较均 P < 0.001），aPWV 为 7.9（6.9-9.1）比 6.1（5.5-6.7）m/s，GlycA 为 850.4（781.3-916.1）比 652.4（581.5-727.1）μmol；GlycB 为 386.1（353.2-426.3）比 310.0（280.5-331.9）μmol/L），GlycA 的 H/W 比为 16.5（14.9-18.1）比 15.0（13.7-16.7），GlycB 的 H/W 比为 5.0（4.6-5.5）比 4.0（3.4-4.3）。此外，aPWV 与 GlycA（r = 0.550）和 GlycB（r = 0.423）浓度以及 GlycA（r = 0.453）和 GlycB（r = 0.510）的 H/W 比值均呈正相关（所有相关均 P < 0.001）。调整潜在混杂因素后，GlycA 浓度（β = 0.212，P < 0.001）和 GlycA（β = 0.150，P = 0.009）和 GlycB（β = 0.155，P = 0.011）的 H/W 比值与 aPWV 独立相关。GlycA 水平检测 aPWV>10 m/s 的个体的 C 统计量为 0.866（95%CI 0.794-0.937），GlycB 的 H/W 比值为 0.862（0.780-0.943）。