sp. nov., isolated from surface water of the Yellow Sea, and proposal of the genus as a member of the family .

A Gram-stain-negative, non-motile and aerobic bacterium, designated HHU G3-2, was isolated from surface water of the Yellow Sea, PR China. Strain HHU G3-2 was positive for oxidase activity and negative for catalase. Optimal growth occurred at 28 °C (range, 20-37 °C), pH 7.0 (range, pH 6.0-9.0) and in the presence of 2-5 % (w/v) NaCl (range, 1-7%). Phylogenetic analysis based on 16S rRNA gene sequences and 120 ubiquitous single-copy protein-coding genes indicated that strain HHU G3-2 formed a distinct phylogenetic lineage with JCM 30134, sharing a 16S rRNA gene sequence similarity of 98.05%. Average nucleotide identity and digital DNA-DNA hybridization values between strain HHU G3-2 and JCM 30134 were 75.74 and 17.80%, respectively, which were below the threshold values of 95-96 and 70 %, respectively. The DNA G+C content of the genomic DNA was 51.17 mol%. The major fatty acids (>10 %) were C 8 (19.8 %), summed feature 3 (C 7/C 6; 15.9 %), summed feature 8 (C 7/C 6; 13.8 %) and C (10.3 %). The predominant isoprenoid quinone was ubiquinone-8. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. Based on the polyphasic taxonomic data, strain HHU G3-2 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is HHU G3-2 (=MCCC 1K04224=JCM 34652=GDMCC 1.2418=CGMCC 1.17397). In addition, we proposed the genus as a member of the family .