Department of Biology, Islamic Azad University of SariBranch, Mazandaran, Iran.
Department of Anatomy, School of Medicine, Tehran University of Medical Science, Tehran, Iran.
Biopreserv Biobank. 2022 Aug;20(4):374-383. doi: 10.1089/bio.2021.0128.
Cryopreservation of spermatogonial stem cells (SSCs) is an important method to restore and maintain fertility in preadolescent children suffering from cancer. For protection of SSCs from cryoinjury, various antioxidant agents have been used. The aim of this study was to assess the antiapoptotic and antioxidant effects of melatonin in frozen-thawed SSCs. SSCs were isolated from testes of neonatal mice (3-6 days old) and their purities were measured by flow cytometry with promyelocytic leukemia zinc finger protein. After culturing, the cells were frozen in two groups (1) control and (2) melatonin (100 μM) and stored for 1 month. Finally, the cell viability, colonization rate, expression of and gene, and intracellular reactive oxygen species (ROS) were evaluated after freezing-thawing. Melatonin increased the viability and colonization of SSCs and gene expression. It also diminished gene expression and intracellular ROS. The results of this study show that melatonin with antioxidant and antiapoptotic effects can be used as an additive for freezing and long-term storage of cells and infertility treatment in the clinic.
精子干细胞 (SSC) 的冷冻保存是一种重要的方法，可以恢复和维持患有癌症的青春期前儿童的生育能力。为了保护 SSC 免受冷冻损伤，已经使用了各种抗氧化剂。本研究旨在评估褪黑素在冷冻-解冻 SSC 中的抗凋亡和抗氧化作用。从新生小鼠（3-6 天龄）的睾丸中分离 SSC，并通过髓系白血病锌指蛋白流式细胞术测量其纯度。培养后，将细胞分为两组（1）对照组和（2）褪黑素（100μM）进行冷冻保存 1 个月。最后，在冷冻-解冻后评估细胞活力、定植率、 基因和 基因的表达以及细胞内活性氧 (ROS)。褪黑素增加了 SSC 的活力和定植率，并 基因的表达。它还减少了 基因的表达和细胞内 ROS。这项研究的结果表明，具有抗氧化和抗凋亡作用的褪黑素可用作冷冻和细胞长期储存以及临床治疗不育症的添加剂。
