McCarrey J R, Hsu K C, Eddy E M, Klevecz R R, Bolen J L
J Exp Zool. 1987 Apr;242(1):107-11. doi: 10.1002/jez.1402420116.
The germ line represents a cell type of unique interest in mammals because it retains complete genotypic totipotency while undergoing significant phenotypic differentiation. Analysis of the mechanism that underlies the maintenance of this totipotency requires the ability to isolate and study all stages of the germ cell lineage. The primordial germ cells (PGC) are the earliest identifiable germ cells in the embryo. It has not previously been possible to isolate PGC in sufficient numbers and purity to facilitate biochemical and/or molecular analysis. We report here that the use of a monoclonal antibody in combination with flow cytometry does permit the isolation of reasonably large and pure yields of viable mouse PGC.
生殖系在哺乳动物中是一种具有独特研究价值的细胞类型，因为它在经历显著的表型分化时仍保留着完整的基因型全能性。分析维持这种全能性的机制需要具备分离和研究生殖细胞谱系所有阶段的能力。原始生殖细胞（PGC）是胚胎中最早可识别的生殖细胞。此前一直无法分离出足够数量和纯度的PGC以利于生化和/或分子分析。我们在此报告，使用单克隆抗体结合流式细胞术确实能够分离出产量可观且纯度较高的存活小鼠PGC。
