Department of Chemistry, College of Sciences, Northeastern University, Box 332, Shenyang, 110819, China.
Department of Chemistry, College of Sciences, Northeastern University, Box 332, Shenyang, 110819, China; Analytical and Testing Center, Northeastern University, Box 106, Shenyang, 110819, China.
Talanta. 2023 Jan 15;252:123825. doi: 10.1016/j.talanta.2022.123825. Epub 2022 Aug 13.
A fluorescence and photothermal dual-signal assay was developed for the determination of ascorbic acid (AA). Cerium/polyacrylic acid modified porphyrin metal-organic frameworks (PCN-224@PAA-Ce MOFs) exhibit oxidase-like activity and can effectively oxidize 3,3 ', 5,5 '-tetramethylbenzidine (TMB) from colorless substrates to blue substrates (oxTMB). The absorption spectrum of oxTMB overlaps with the emission spectrum of PCN-224@PAA-Ce, resulting in quenching of fluorescence emission of PCN-224@PAA-Ce. oxTMB, as an excellent photothermal agent, converts the optical signal into the thermal signal (temperature increasing) driven by 808 nm laser. In the presence of AA, the blue oxTMB was reduced to colorless TMB, which led to the fluorescence recovery of PCN-224@PAA-Ce and at the same time, the photothermal signal was changed with the temperature decreasing under the driving of 808 nm laser. The detection limits (LOD) of fluorescence/photothermal dual-mode AA sensor strategies were as low as 0.73 μM and 1.4 μM, respectively. Rapid analysis of ascorbic acid was accomplished with the present procedure, AA in vitamin C tablets and human serum samples were validated.
开发了一种用于测定抗坏血酸 (AA) 的荧光和光热双信号测定法。铈/聚丙烯酸修饰的卟啉金属有机骨架 (PCN-224@PAA-Ce MOFs) 具有氧化酶样活性，可以有效地将无色底物 3,3',5,5'-四甲基联苯胺 (TMB) 氧化为蓝色底物 (oxTMB)。oxTMB 的吸收光谱与 PCN-224@PAA-Ce 的发射光谱重叠，导致 PCN-224@PAA-Ce 的荧光发射猝灭。oxTMB 作为一种优良的光热试剂，将光学信号转换为 808nm 激光驱动的热信号（温度升高）。在 AA 的存在下，蓝色 oxTMB 被还原为无色 TMB，导致 PCN-224@PAA-Ce 的荧光恢复，同时在 808nm 激光驱动下，光热信号随温度降低而变化。荧光/光热双模 AA 传感器策略的检测限 (LOD) 分别低至 0.73μM 和 1.4μM。本方法可快速分析抗坏血酸，验证了维生素 C 片剂和人血清样品中的 AA。
