School of Civil and Environmental Engineering, University of Technology Sydney, Sydney, NWS 2007, Australia.
School of Civil and Environmental Engineering, University of Technology Sydney, Sydney, NWS 2007, Australia; Institute of Environmental Sciences, Nguyen Tat Thanh University, Ho Chi Minh City, Viet Nam; Joint Research Center for Protective Infrastructure Technology and Environmental Green Bioprocess, School of Environmental and Municipal Engineering, Tianjin Chengjian University, Tianjin 300384, China.
Sci Total Environ. 2022 Dec 10;851(Pt 2):158112. doi: 10.1016/j.scitotenv.2022.158112. Epub 2022 Aug 17.
Organic-rich wastewater is a feasible feedstock for biohydrogen production. Numerous review on the performance of microorganisms and the diversity of their communities during a biohydrogen process were published. However, there is still no in-depth overview of enzymes for biohydrogen production from wastewater and their scale-up applications. This review aims at providing an insightful exploration of critical discussion in terms of: (i) the roles and applications of enzymes in wastewater-based biohydrogen fermentation; (ii) systematical introduction to the enzymatic processes of photo fermentation and dark fermentation; (iii) parameters that affect enzymatic performances and measures for enzyme activity/ability enhancement; (iv) biohydrogen production bioreactors; as well as (v) enzymatic biohydrogen production systems and their larger scales application. Furthermore, to assess the best applications of enzymes in biohydrogen production from wastewater, existing problems and feasible future studies on the development of low-cost enzyme production methods and immobilized enzymes, the construction of multiple enzyme cooperation systems, the study of biohydrogen production mechanisms, more effective bioreactor exploration, larger scales enzymatic biohydrogen production, and the enhancement of enzyme activity or ability are also addressed.
富含有机物的废水是生产生物氢气的一种可行原料。大量关于微生物在生物制氢过程中的性能和多样性的综述已经发表。然而，对于从废水中生产生物氢气的酶及其放大应用，仍然没有深入的综述。本综述旨在提供对以下方面的深入探讨：（i）酶在基于废水的生物氢气发酵中的作用和应用；（ii）光发酵和暗发酵的酶促过程的系统介绍；（iii）影响酶性能的参数和提高酶活性/能力的措施；（iv）生物氢气生产生物反应器；以及（v）酶法生物氢气生产系统及其更大规模的应用。此外，为了评估酶在废水生物制氢中的最佳应用，需要对开发低成本酶生产方法和固定化酶、构建多种酶协同系统、研究生物制氢机制、更有效地探索生物反应器、进行更大规模的酶法生物制氢以及提高酶活性或能力等方面进行现有问题和可行的未来研究。
