Department of Pathology, Gyeongsang National University Changwon Hospital, Changwon, Republic of Korea.
lnstitute of Health Sciences, Gyeongsang National University School of Medicine, Jinju, Republic of Korea.
Cancer Genomics Proteomics. 2022 Sep-Oct;19(5):606-613. doi: 10.21873/cgp.20345.
BACKGROUND/AIM: RAB27A and RAB27B are involved in exosome secretion. To date, there have been many attempts to elucidate the roles of RAB27A and RAB27B in the prognosis of various cancer types. The association of RAB27A and RAB27B expression with the clinical and pathological features was evaluated in patients with stomach cancer.
A total of 360 patients who had undergone surgery for stomach cancer between January 1999 and December 2007 at Gyeongsang National University were enrolled in the study. Disease-free survival (DFS) and disease-specific survival (DSS) were compared according to immunohistochemistry of tumor samples. RAB27A and RAB27B mRNA and protein were also extracted from four stomach cancer cell lines using quantitative polymerase chain reaction and western blotting.
Strong nuclear RAB27A expression in tumor samples was statistically significantly correlated with lymph node metastasis. Cytoplasmic RAB27B expression was related to poor disease-free survival and its combined cytoplasmic and membranous expression was related to disease-specific survival of patients with different histopathological types of stomach cancer. High RAB27A expression and high RAB27B expression was found in four stomach cancer cell blocks. Among the four cell lines, NCI-N87 exhibited the lowest relative mRNA density and HS746T exhibited the highest relative protein density for both RAB27A and RAB27B.
RAB27A and RAB27B expression may help predict lymph node metastasis and survival of patients with gastric cancer.
背景/目的：RAB27A 和 RAB27B 参与外体分泌。迄今为止，已有许多尝试旨在阐明 RAB27A 和 RAB27B 在各种癌症类型预后中的作用。本研究评估了 RAB27A 和 RAB27B 表达与胃癌患者临床病理特征的关系。
本研究共纳入 1999 年 1 月至 2007 年 12 月期间在全南国立大学接受胃癌手术的 360 例患者。根据肿瘤样本的免疫组织化学，比较无病生存（DFS）和疾病特异性生存（DSS）。使用定量聚合酶链反应和蛋白质印迹从 4 种胃癌细胞系中提取 RAB27A 和 RAB27B mRNA 和蛋白质。
肿瘤样本中 RAB27A 的强核表达与淋巴结转移呈统计学显著相关。细胞质 RAB27B 的表达与无病生存不良相关，其细胞质和膜结合表达与不同组织病理学类型胃癌患者的疾病特异性生存相关。在 4 个胃癌细胞块中发现高 RAB27A 表达和高 RAB27B 表达。在这 4 个细胞系中，NCI-N87 细胞系的 RAB27A 和 RAB27B 的相对 mRNA 密度最低，而 HS746T 细胞系的相对蛋白密度最高。
RAB27A 和 RAB27B 的表达可能有助于预测胃癌患者的淋巴结转移和生存。
