Wang Shengyi, Wang Peng, Zhang Ruzhi
Department of Dermatology, The Third Affiliated Hospital of Soochow University, Changzhou, China.
Xuzhou Central Hospital, Xuzhou, Jiangsu, China.
Cell Tissue Bank. 2023 Mar;24(1):253-264. doi: 10.1007/s10561-022-10031-7. Epub 2022 Aug 20.
To investigate the effect of human adipose tissue-derived multilineage-differentiating stress-enduring (Muse) cells on the oxidative stress injury of human epidermal melanocytes (HEMs) in vitro. HEMs were treated with HO to establish an oxidative stress injury model and then were co-cultured with adipose tissue-derived Muse cells. Immunohistochemistry, flow cytometry and Western blotting were used to assess changes in autophagy flux, apoptosis, expression of melanin synthesis related proteins and proliferation of melanocytes. Our findings demonstrate that co-culture with Muse cells significantly increased the tolerance of HEMs to oxidative stress, enhanced autophagy flux and reduced apoptosis. The expression of proteins related to the formation of melanin increased as did cell proliferation. Treatment with the autophagy inhibitor, 3-methyladenine (3MA), partially counteracted the improvement of oxidative stress tolerance in melanocytes elicited by co-culture with Muse cells. Muse cells promote autophagy and oxidative stress tolerance of melanocytes.
为了在体外研究人脂肪组织来源的多向分化应激耐受（Muse）细胞对人表皮黑素细胞（HEMs）氧化应激损伤的影响。用HO处理HEMs以建立氧化应激损伤模型，然后将其与脂肪组织来源的Muse细胞共培养。采用免疫组织化学、流式细胞术和蛋白质印迹法评估自噬通量、细胞凋亡、黑色素合成相关蛋白表达以及黑素细胞增殖的变化。我们的研究结果表明，与Muse细胞共培养显著提高了HEMs对氧化应激的耐受性，增强了自噬通量并减少了细胞凋亡。与黑色素形成相关的蛋白质表达增加，细胞增殖也增加。用自噬抑制剂3-甲基腺嘌呤（3MA）处理，部分抵消了与Muse细胞共培养引起的黑素细胞氧化应激耐受性的改善。Muse细胞促进黑素细胞的自噬和氧化应激耐受性。
