人胚干细胞系 NCHi006-A 源自一名左心发育不全综合征（HLHS）患者的特征描述。
Characterization of an iPSC line NCHi006-A from a patient with hypoplastic left heart syndrome (HLHS).
机构信息
Center for Cardiovascular Research, Abigail Wexner Research Institute, Nationwide Children's Hospital, Columbus, OH, USA; The Heart Center, Nationwide Children's Hospital, Columbus, OH, USA.
Center for Cardiovascular Research, Abigail Wexner Research Institute, Nationwide Children's Hospital, Columbus, OH, USA; The Heart Center, Nationwide Children's Hospital, Columbus, OH, USA; Department of Pediatrics, The Ohio State University College of Medicine, Columbus, OH, USA.
出版信息
Stem Cell Res. 2022 Oct;64:102892. doi: 10.1016/j.scr.2022.102892. Epub 2022 Aug 9.
Hypoplastic left heart syndrome (HLHS) is a severe congenital heart defect characterized by underdeveloped structures on the left side of the heart, including hypoplasia of the left ventricle and stenosis or atresia of the aortic and mitral valves. Here, we generated an iPSC line from the peripheral blood mononuclear cells of a male patient with HLHS through Sendai virus-mediated transfection of 4 Yamanaka factors. This iPSC line exhibited normal morphology, expressed pluripotency markers, had a normal karyotype, and could differentiate into cells of three germ layers. This iPSC line can be used for studying cellular and developmental etiologies of HLHS.
左心发育不全综合征（HLHS）是一种严重的先天性心脏缺陷，其特征是心脏左侧结构发育不全，包括左心室发育不良以及主动脉瓣和二尖瓣狭窄或闭锁。在这里，我们通过 Sendai 病毒介导的 4 个 Yamanaka 因子转染，从 HLHS 男性患者的外周血单核细胞中生成了一个 iPSC 系。该 iPSC 系表现出正常的形态，表达多能性标志物，具有正常的核型，并能分化为三个胚层的细胞。该 iPSC 系可用于研究 HLHS 的细胞和发育病因。
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