Department of Biotechnology, Faculty of Science, Selcuk University, Selcuklu, 42130, Konya, Turkey.
Department of Molecular Biology and Genetics, Faculty of Science, Necmettin Erbakan University, Meram, 42090, Konya, Turkey.
Chemosphere. 2022 Nov;307(Pt 4):136048. doi: 10.1016/j.chemosphere.2022.136048. Epub 2022 Aug 17.
The ever-increasing plastic pollution in soil and water resources raises concerns about its effects on terrestrial plants and agroecosystems. Although there are many reports about the contamination with nanoplastics on plants, the presence of magneto-assisted nanomaterials enabling the removal of their adverse impacts still remains unclear. Therefore, the purpose of the current study is to evaluate the potential of nanomaterial FeO-modified graphene oxide (FGO, 50-250 mg L) to eliminate the adverse effects of nanoplastics in plants. Wheat plants exposed to polystyrene nanoplastics concentrations (PS, 10, 50 and 100 mg L) showed decreased growth, water content and loss of photosynthetic efficiency. PS toxicity negatively altered gas exchange, antenna structure and electron transport in photosystems. Although the antioxidant system was partially activated (only superoxide dismutase (SOD), NADPH oxidase (NOX) and glutathione reductase (GR)) in plants treated with PS, it failed to prevent PS-triggered oxidative damage, as showing lipid peroxidation and hydrogen peroxide (HO) levels. FGOs eliminated the adverse impacts of PS pollution on growth, water status, gas exchange and oxidative stress markers. In addition, FGOs preserve the biochemical reactions of photosynthesis by actively increasing chlorophyll fluorescence parameters in the stressed-wheat leaves. The activities of all enzymatic antioxidants increased, and the HO and TBARS contents decreased. GSH-mediated detoxifying antioxidants such as glutathione S-transferase (GST) and glutathione peroxidase (GPX) were stimulated by FGOs against PS pollution. FGOs also triggered the enzymes and non-enzymes related to the Asada-Halliwell cycle and protected the regeneration of ascorbate (AsA) and glutathione (GSH). Our findings indicated that FGO had the potential to mitigate nanoplastic-induced damage in wheat by regulating water relations, protecting photosynthesis reactions and providing efficient ROS scavenging with high antioxidant capacity. This is the first report on removing PS-induced damage by FGO applications in wheat leaves.
土壤和水资源中不断增加的塑料污染引起了人们对其对陆地植物和农业生态系统影响的关注。尽管有许多关于植物中纳米塑料污染的报道，但能够去除其不利影响的磁辅助纳米材料的存在仍然不清楚。因此，本研究的目的是评估纳米材料 FeO 修饰氧化石墨烯（FGO，50-250mg/L）消除植物中纳米塑料不利影响的潜力。暴露于聚苯乙烯纳米塑料浓度（PS，10、50 和 100mg/L）的小麦植物表现出生长、水分含量和光合效率降低。PS 毒性对气体交换、天线结构和光系统中的电子传递产生负面影响。尽管用 PS 处理的植物部分激活了抗氧化系统（仅超氧化物歧化酶（SOD）、NADPH 氧化酶（NOX）和谷胱甘肽还原酶（GR）），但它未能防止 PS 引发的氧化损伤，如脂质过氧化和过氧化氢（HO）水平。FGO 消除了 PS 污染对生长、水分状况、气体交换和氧化应激标志物的不利影响。此外，FGO 通过积极增加胁迫小麦叶片中的叶绿素荧光参数来维持光合作用的生化反应。所有酶促抗氧化剂的活性增加，HO 和 TBARS 含量降低。谷胱甘肽介导的解毒抗氧化剂，如谷胱甘肽 S-转移酶（GST）和谷胱甘肽过氧化物酶（GPX），在 FGO 存在下受到 PS 污染的刺激。FGO 还触发了与 Asada-Halliwell 循环相关的酶和非酶，并保护了抗坏血酸（AsA）和谷胱甘肽（GSH）的再生。我们的研究结果表明，FGO 通过调节水分关系、保护光合作用反应和提供高效的 ROS 清除作用以及具有高抗氧化能力来减轻小麦中纳米塑料引起的损伤，具有减轻纳米塑料诱导损伤的潜力。这是 FGO 在小麦叶片中去除 PS 诱导损伤的首次报道。
