Ribeiro Rafael Antonio de Oliveira, Peruchi Victória, Fernandes Lídia de Oliveira, Anselmi Caroline, Soares Igor Paulino Mendes, Hebling Josimeri, Costa Carlos Alberto de Souza
Department of Dental Materials and Prosthodontics, School of Dentistry, São Paulo State University (UNESP), Araraquara, Brazil.
Department of Restorative Dentistry, School of Dentistry, São Paulo State University (UNESP), Araraquara, Brazil.
Photodiagnosis Photodyn Ther. 2022 Dec;40:103069. doi: 10.1016/j.pdpdt.2022.103069. Epub 2022 Aug 17.
To assess the potential influence of violet LED (V-LED) application time on the esthetic efficacy and cytotoxicity of a 35% HO bleaching gel.
Stained and standardized enamel/dentin discs were subjected to one in-office tooth bleaching session (45 min), and the gel was either irradiated or not with V-LED. Thus, the following groups were established (n = 8): G1: No treatment (negative control, NC); G2: 35% HO (positive control, PC); G3: 35%HO + V-LED/15 min; G4: 35%HO + V-LED/30 min; G5: 35%HO + V-LED/45 min. First, esthetic efficacy was assessed (ΔE and ΔWI). Discs assembled in artificial pulp chambers were subjected to the same bleaching treatments. Then, the extracts (culture medium + diffused bleaching gel components) were collected and applied to MDPC-23 pulp cells, which were analyzed for viability (Live/Dead, MTT) and oxidative stress (OxS). The amount of HO in the extracts was also determined (leuco crystal-violet/peroxidase). The data were subjected to ANOVA/Tukey at a 5% significance level.
Although esthetic efficacy did not differ among the irradiated groups (G3, G4, and G5) (p > 0.05), their results were higher than in G2 (PC; p < 0.05). In the irradiated groups, the cell viability and OxS as well as the amount of HO in the extracts were statistically similar to G2 (PC), regardless of irradiation time (p > 0.05).
Although V-LED improves the esthetic outcome of in-office tooth bleaching, increasing irradiation time does not effect the color changes and cytotoxicity of this professional therapy.
评估紫光发光二极管（V-LED）照射时间对35%过氧化氢（HO）漂白凝胶的美学效果和细胞毒性的潜在影响。
将染色并标准化的牙釉质/牙本质圆盘进行一次诊室牙齿漂白治疗（45分钟），凝胶用V-LED照射或不照射。因此，设立了以下几组（n = 8）：G1：不治疗（阴性对照，NC）；G2：35% HO（阳性对照，PC）；G3：35% HO + V-LED/15分钟；G4：35% HO + V-LED/30分钟；G5：35% HO + V-LED/45分钟。首先，评估美学效果（ΔE和ΔWI）。将组装在人工牙髓腔中的圆盘进行相同的漂白处理。然后，收集提取物（培养基 + 扩散的漂白凝胶成分）并应用于MDPC-23牙髓细胞，分析其活力（活/死，MTT）和氧化应激（OxS）。还测定了提取物中HO的含量（无色结晶紫/过氧化物酶）。数据在5%显著性水平下进行方差分析/ Tukey检验。
尽管照射组（G3、G4和G5）之间的美学效果没有差异（p > 0.05），但其结果高于G2组（PC；p < 0.05）。在照射组中，无论照射时间如何，细胞活力、OxS以及提取物中HO的含量与G2组（PC）在统计学上相似（p > 0.05）。
尽管V-LED可改善诊室牙齿漂白的美学效果，但增加照射时间并不影响这种专业治疗的颜色变化和细胞毒性。
