Department of Horticultural Sciences, Faculty of Agriculture, Ilam University, P.O. Box 69315-516, Ilam, Iran.
Department of Physics, Faculty of Science, Vali-e-Asr University of Rafsanjan, Rafsanjan, Iran.
Sci Rep. 2022 Aug 20;12(1):14232. doi: 10.1038/s41598-022-18642-6.
The rapid yellowing of the leaves on cut flowers with leafy stems severely limits their vase life and commercial value. In this study, the effect of a composite of multi-walled carbon nanotubes (MWCNTs) and polyvinyl pyrrolidone (PVP) on the longevity of cut Alstroemeria flowers (Alstroemeria hybrida) was investigated to obtain a solution to this problem. A range of MWCNTs/PVP composite concentrations (0, 3, 6, and 9 mg L) was applied in a vase solution (for 24 h) as pulse treatments. Our findings indicate that the composite of MWCNTs and PVP exhibits excellent dispersibility in a vase solution. The results demonstrate that a 3 mg L MWCNTs/PVP concentration was the most effective, extending the vase life of cut Alstroemeria flowers by up to 27 days. Pulsing with MWCNTs/PVP delayed the onset of floret abscission and leaf yellowing by 5 and 18 days, respectively. Additionally, when MWCNTs/PVP solution was applied to cut stems, water uptake remained consistently greater than that of the control. Additionally, MWCNTs/PVP increased the total chlorophyll content, soluble protein content, and POX enzyme activity of leaves while decreasing the malondialdehyde (MDA) content. The results indicate that this composite exhibited antimicrobial activity against gram-positive and -negative bacteria, particularly at a concentration of 3 mg L. This study demonstrated that adding MWCNTs/PVP to a vase solution of Alstroemeria cut flowers increased their longevity with minimal leaf yellowing symptoms compared to untreated cut stems. As a result, this nanocomposite can be used safely and effectively in vase solutions and in combination with other preservatives.
切花叶片迅速变黄会严重限制其瓶插寿命和商业价值。本研究旨在通过添加多壁碳纳米管（MWCNTs）和聚乙烯吡咯烷酮（PVP）的复合物，来解决这一问题，以延长切花六出花（Alstroemeria hybrida）的瓶插寿命。我们将一系列不同浓度的 MWCNTs/PVP 复合物（0、3、6 和 9mg/L）应用于瓶插液（24h）中，作为脉冲处理。结果表明，MWCNTs 和 PVP 的复合物在瓶插液中具有良好的分散性。结果表明，浓度为 3mg/L 的 MWCNTs/PVP 效果最佳，可将切花六出花的瓶插寿命延长 27 天。MWCNTs/PVP 脉冲处理分别可将小花脱落和叶片黄化的起始时间推迟 5 天和 18 天。此外，当 MWCNTs/PVP 溶液应用于切花茎时，水分吸收量始终大于对照组。MWCNTs/PVP 增加了叶片中的总叶绿素含量、可溶性蛋白含量和过氧化物酶（POX）活性，同时降低了丙二醛（MDA）含量。结果表明，该复合物对革兰氏阳性菌和阴性菌均具有抗菌活性，尤其是在 3mg/L 的浓度下。本研究表明，与未处理的切花茎相比，在六出花瓶插液中添加 MWCNTs/PVP 可在最小化叶片黄化症状的情况下，延长切花的瓶插寿命。因此，这种纳米复合材料可安全有效地用于瓶插液中，并与其他防腐剂结合使用。
