Department of Spine Surgery, Qingdao Municipal Hospital, Qingdao, 266000, China.
Department of Examination Center, Qingdao Municipal Hospital, Qingdao, 266000, China.
Cell Mol Biol (Noisy-le-grand). 2022 Mar 31;68(3):282-293. doi: 10.14715/cmb/2022.68.3.31.
Intervertebral disc degeneration (IDD) is a common clinical symptom of multifactorial disease. The treatment and expenditure of IDD cause huge economic and psychological harm to patients, and there is no root treatment in the clinic. However, the appearance of tissue engineering materials provides a new idea for the treatment of early IDD. KLD-12 polypeptide material is a new kind of polypeptide scaffold material, which can be used to repair early IDD and TGF-β1Transforming growth factor-1 plays an important role in the proliferation of Intervertebral disc cells and inhibition of inflammatory response. In order to further understand the tissue engineering material kld-12 polypeptide / TGF-β1 the biomechanical properties of nanofiber gel, and to clarify tissue engineering material KLD-12 polypeptide TGF-β1nanofiber gel provides an experimental basis for the protection and mechanism of early IDD. In this paper, tissue engineering material KLD-12 polypeptide /TGF-β1 is mainly studied as the protective effect and mechanism of nanofiber gel on early IDD. In this paper, through the study of the tissue structure of the intervertebral disc, the composition of the nucleus pulposus, annulus fibrosus and cartilage endplate was studied. The objective was to study the relationship between transforming growth factor TGF-β1 and IDD and to understand its important role in the proliferation of intervertebral disc cells and inhibition of inflammatory response. In this paper, we studied the molecular basis of IDD, the main reason is the imbalance of extracellular matrix synthesis and degradation of Intervertebral disc cells, to understand the structural characteristics of cartilage endplate and the composition of Intervertebral disc fibroblasts. In this study, we studied the cell proliferation activity, the ratio of surviving dead cells, the content of glucosaminoglycans, the content of polyproteoglycan and type II collagen in the gel, and studied the protective effect and mechanism of tissue engineering material KLD-12 polypeptide /TGF-β1 nanofiber gel on early IDD. The results showed that kld-12 polypeptide / TGF-β1 was more effective in the proliferation activity of annulus fibrosus cells of nanofiber gel is higher than that of KLD-12 polypeptide/annulus fibroin nanofiber gel. On 2d, the difference in cell proliferation activity was not obvious, KLD-12 polypeptide / TGF- β 1 the fibrous annulus cell proliferation activity of nanofiber gel was 0.796, and the proliferation activity of KLD-12 polypeptide/annulus fibroin nanofiber gel was 0.786. On the 14d, KLD-12 polypeptide / TGF- β 1 the fibrous annulus cell proliferation activity of nanofiber gel was 1.204, and the proliferation activity of KLD-12 polypeptide/annulus fibroin nanofiber gel was 1.034.
椎间盘退变（IDD）是一种常见的多因素疾病的临床症状。IDD 的治疗和支出给患者带来了巨大的经济和心理伤害，临床上没有根治方法。然而，组织工程材料的出现为早期 IDD 的治疗提供了新的思路。KLD-12 多肽材料是一种新型的多肽支架材料，可用于修复早期 IDD 和 TGF-β1。转化生长因子-β1 在椎间盘细胞的增殖和抑制炎症反应中起着重要作用。为了进一步了解组织工程材料 KLD-12 多肽/TGF-β1纳米纤维凝胶的生物力学性能，并阐明组织工程材料 KLD-12 多肽/TGF-β1纳米纤维凝胶对早期 IDD 的保护和机制，为早期 IDD 的保护和机制提供实验基础。本文主要研究组织工程材料 KLD-12 多肽/TGF-β1纳米纤维凝胶对早期 IDD 的保护作用及其机制。本文通过对椎间盘组织结构、髓核、纤维环和软骨终板组成的研究，探讨转化生长因子 TGF-β1 与 IDD 的关系，了解其在椎间盘细胞增殖和抑制炎症反应中的重要作用。本文研究了 IDD 的分子基础，主要原因是椎间盘细胞外基质合成和降解失衡，了解软骨终板的结构特征和椎间盘成纤维细胞的组成。在本研究中，我们研究了细胞增殖活性、存活死细胞比例、糖胺聚糖含量、多聚蛋白聚糖含量和 II 型胶原含量，研究了组织工程材料 KLD-12 多肽/TGF-β1 纳米纤维凝胶对早期 IDD 的保护作用及其机制。结果表明，KLD-12 多肽/TGF-β1 在纳米纤维凝胶中对纤维环细胞增殖活性的作用优于 KLD-12 多肽/纤维环蛋白纳米纤维凝胶。在 2d 时，细胞增殖活性的差异不明显，KLD-12 多肽/TGF-β1 纳米纤维凝胶中纤维环细胞增殖活性为 0.796，KLD-12 多肽/纤维环蛋白纳米纤维凝胶的增殖活性为 0.786。在 14d 时，KLD-12 多肽/TGF-β1 纳米纤维凝胶中纤维环细胞增殖活性为 1.204，KLD-12 多肽/纤维环蛋白纳米纤维凝胶的增殖活性为 1.034。
