Department of Emergency Ward, Hai'an People's Hospital Affiliated to Nantong University, Nantong 226699, China.
Cell Mol Biol (Noisy-le-grand). 2022 Mar 31;68(3):140-148. doi: 10.14715/cmb/2022.68.3.17.
The repairing effect of new dexamethasone nanoparticles in the treatment of acute lung injury was investigated in this study, as well as cluster nursing. In this study, new dexamethasone model drugs were prepared by the aqueous solvent diffusion method, such as anti-ICAM-I monoclonal antibody-modified anionic dexamethasone NLCs and anti-ICAM-I monoclonal antibody-modified cationic dexamethasone NLCs. Besides, the physical and chemical properties and repairing effects of cationic dexamethasone on acute lung injury were compared. A mouse model of acute lung injury was established, and the anti-inflammatory effect of dexamethasone was evaluated by intravenous injection of dexamethasone nanoparticles in the intervention group and normal healthy mice in the control group. A total of 100 patients with acute lung injury in Hai'an People's Hospital Affiliated with Nantong University were selected, of which 50 cases were given cluster nursing intervention and the other 50 cases were taken as the control group. A human vascular endothelial cell line was applied to establish the model cells, and a model of inflammatory endothelial cells in acute lung injury was constructed using lipopolysaccharide stimulation, to verify the cytotoxicity of dexamethasone NLCs. It was found that the anion particle size was 250.12 ± 20.15 nm, the cationic particle size was 245.7 ± 2.1 nm; their Zeta potentials were -31 ± 0.5 mV and 38 ± 0.6 mV in turn; their encapsulation rates were 91% and 83%, respectively; the drug loading was 3.7% and 3.4% in sequence; the release lowest rate was 60%. The 50% lethal dose of anionic cells was higher than 600 g/mL, while that of cationic cells was lower. The respiratory function of the cluster nursing intervention group was better markedly than that of the control group, and the lung infection rate was 2.5% in the intervention group and 15% in the control group. In conclusion, dexamethasone nanoparticles had good anti-inflammatory effects. Anionic ICAM NLCs were less toxic than cationic cells and could better bind to lung vascular endothelial cells, which might reduce adverse drug reactions. Therefore, the building of bundled nursing could effectively alleviate respiratory dysfunction and reduce the infection rate of patients.
本研究考察了新型地塞米松纳米粒在治疗急性肺损伤中的修复作用，并进行了集束化护理。本研究采用水相溶剂扩散法制备新型地塞米松模型药物，如抗 ICAM-I 单克隆抗体修饰的阴离子地塞米松 NLCs 和抗 ICAM-I 单克隆抗体修饰的阳离子地塞米松 NLCs。此外，还比较了阳离子地塞米松对急性肺损伤的物理化学性质和修复作用。建立了小鼠急性肺损伤模型，通过静脉注射地塞米松纳米粒对干预组和对照组正常健康小鼠进行抗炎作用评价。选择南通大学附属海安人民医院 100 例急性肺损伤患者，其中 50 例给予集束化护理干预，另 50 例作为对照组。应用人血管内皮细胞系建立模型细胞，采用脂多糖刺激构建急性肺损伤炎症内皮细胞模型，验证地塞米松 NLCs 的细胞毒性。结果表明，阴离子粒径为 250.12±20.15nm，阳离子粒径为 245.7±2.1nm；其 Zeta 电位分别为-31±0.5mV 和 38±0.6mV；包封率分别为 91%和 83%；载药量分别为 3.7%和 3.4%；最低释放率为 60%。阴离子细胞的 50%致死剂量高于 600μg/ml，而阳离子细胞的 50%致死剂量则较低。集束化护理干预组的呼吸功能明显优于对照组，干预组的肺部感染率为 2.5%，对照组为 15%。综上所述，地塞米松纳米粒具有良好的抗炎作用。阴离子 ICAM NLCs 的毒性小于阳离子细胞，能更好地与肺血管内皮细胞结合，可能减少药物不良反应。因此，集束化护理的建立能有效缓解呼吸功能障碍，降低患者感染率。
