Department of Chemical Engineering, Queen's University, Kingston K7L 3N6, Canada.
Department of Chemical Engineering, Queen's University, Kingston K7L 3N6, Canada.
Carbohydr Polym. 2022 Nov 1;295:119850. doi: 10.1016/j.carbpol.2022.119850. Epub 2022 Jul 9.
Three process variations are developed to synthesize dextran-bovine serum albumin (BSA) model conjugate through the Maillard reaction and a Schiff base-Amadori solid phase rearrangement mechanism at 90 °C. The influences of lyophilization pH and dextran:BSA molar ratio on the reaction are investigated, with the highest yields achieved by absorbing heat evenly and continuously removing the water by-product under vacuum. Lyophilizing at pH 7.8 provides higher yields than materials lyophilized at pH 5.9, a result attributed to higher reactivity of deprotonated amine at pH 7.8. The purified dextran-BSA conjugate solution is heated to above the BSA denaturation temperature (80 °C) at pH 5.2 to form nanogels with a hydrodynamic diameter of 195-400 nm, with the size dependent on the conjugate composition estimated by bicinchoninic acid protein and glycoprotein carbohydrate assays.
三种工艺变化被开发出来，通过美拉德反应和席夫碱-阿马多里固相重排机制，在 90°C 下合成葡聚糖-牛血清白蛋白（BSA）模型缀合物。研究了冻干 pH 值和葡聚糖：BSA 摩尔比对反应的影响，通过在真空下均匀吸收热量和连续去除水副产物，实现了最高产率。在 pH 7.8 下冻干比在 pH 5.9 下冻干提供更高的产率，这归因于 pH 7.8 下脱质子化胺的更高反应性。将纯化的葡聚糖-BSA 缀合物溶液在 pH 5.2 下加热至高于 BSA 变性温度（80°C），以形成水动力学直径为 195-400nm 的纳米凝胶，其尺寸取决于通过二喹啉甲酸蛋白和糖蛋白碳水化合物测定估计的缀合物组成。
