Effects of adrenal steroids on Na transport in the lower intestine (coprodeum) of the hen.

The influence of adrenal steroids on sodium transport in hen coprodeum was investigated by electrophysiological methods. Laying hens were maintained on low-NaCl diet (LS), or on high-NaCl diet (HS). HS hens were pretreated with aldosterone (128 micrograms/kg) or dexamethasone (1 mg/kg) before experiment. A group of LS hens received spironolactone (70 or 160 mg/kg, for three days). The effects of these dietary and hormonal manipulations on the amiloride-sensitive part of the short-circuit current were examined. This part is in excellent agreement with the net Na flux, and therefore a direct electrical measurement for Na transport. After depolarizing the basolateral membrane potential with a high K concentration, the apical Na permeability and the intracellular Na activity were investigated by current-voltage relations for the different experimental conditions. Plasma aldosterone concentrations (PA) were low in HS hens, dexamethasone-treated HS hens and spironolactone-treated LS hens (less than 70 pM). In contrast LS hens and aldosterone-treated HS hens had a PA concentration of 596 +/- 70 and 583 +/- 172 pM, respectively. LS diet (chronic stimulation) had the largest stimulatory effect on Na transport and apical Na permeability. Hormone-treated animals had three- to fourfold lower values. Spironolactone supply in LS hens decreased Na transport and apical Na permeability about 50%. The results provide evidence that both mineralo- and gluco-corticoids stimulate Na transport in this tissue by increasing the apical Na permeability. Quantitative differences between acute and chronic stimulation reveal a secondary slower adaptation in apical membrane properties.