Programa de Pós-Graduação Multicêntrico em Ciências Fisiológicas-SBFis, Department of Basic Sciences, School of Dentistry, São Paulo State University (UNESP), Araçatuba, Brazil.
Department of Preventive and Restorative Dentistry, School of Dentistry, São Paulo State University (UNESP), Araçatuba, Brazil.
J Periodontol. 2023 Apr;94(4):487-497. doi: 10.1002/JPER.22-0139. Epub 2022 Sep 25.
Maternal periodontal disease (PED) and apical periodontitis (AP) are associated insulin resistance (IR), increased tumor necrosis factor-α (TNF-α) levels, and alterations in insulin signaling (IS) in the gastrocnemius muscle (GM) of adult offspring. TNF-α stimulates I kappa B kinase (IKK) and c-Jun N-terminal protein kinase (JNK), resulting in IS attenuation. However, studies that investigated the maternal true endodontic-periodontal lesion (EPL) in offspring are scarce, and in this case, the impact could be even higher. This study aimed to evaluate the effects of EPL on the IR, IS, and inflammatory pathways on the offspring GM.
Female Wistar rats were distributed into control, AP, PED, and EPL groups. After 30 days of oral inflammation induction, rats from all groups were allowed to mate with healthy rats. The body weight of the offspring was assessed from birth to 75 days of age. After 75 days, the following measurements were performed: glycemia, insulinemia, IR, TNF-α content, and IKKα/β, JNK, pp185 (Tyr), and IRS-1 (Ser) phosphorylation status in the GM.
Maternal PED and EPL were associated with low birth weights. All maternal oral inflammations promoted IR and IS impairment in the GM and only maternal PED and EPL caused an increase in TNF-α content and IKKα/β phosphorylation status in the GM of offspring. The offspring of the rats with EPL group showed worsening of metabolic changes when compared with offspring of rats with AP or PED.
Association of maternal AP and PED promoted a more pronounced worsening in the health of the adult offspring.
母体牙周病（PED）和根尖周炎（AP）与胰岛素抵抗（IR）、肿瘤坏死因子-α（TNF-α）水平升高以及腓肠肌（GM）胰岛素信号转导（IS）改变有关。TNF-α刺激 IκB 激酶（IKK）和 c-Jun N-末端蛋白激酶（JNK），导致 IS 衰减。然而，研究母体真正的牙髓-牙周病变（EPL）对后代的影响的研究很少，而且在这种情况下，影响可能更大。本研究旨在评估 EPL 对后代 GM 中 IR、IS 和炎症途径的影响。
将雌性 Wistar 大鼠分为对照组、AP 组、PED 组和 EPL 组。在口腔炎症诱导 30 天后，所有组的大鼠都允许与健康大鼠交配。从出生到 75 天龄，评估后代的体重。75 天后，进行以下测量：血糖、胰岛素血症、IR、GM 中的 TNF-α含量以及 IKKα/β、JNK、pp185（Tyr）和 IRS-1（Ser）磷酸化状态。
母体 PED 和 EPL 与低出生体重有关。所有母体口腔炎症均导致 GM 中 IR 和 IS 受损，只有母体 PED 和 EPL 导致 GM 中 TNF-α含量和 IKKα/β磷酸化状态增加。与 AP 或 PED 大鼠的后代相比，EPL 组大鼠的后代表现出代谢变化的恶化。
母体 AP 和 PED 的联合作用导致成年后代的健康状况更明显恶化。
