Department of Biochemistry and Molecular Biology, University of Southern Denmark, 5230 Odense, Denmark.
Department of Cancer and Inflammation Research, Institute for Molecular Medicine, University of Southern Denmark, 5230 Odense, Denmark.
Eur J Pharm Sci. 2022 Nov 1;178:106282. doi: 10.1016/j.ejps.2022.106282. Epub 2022 Aug 19.
Rat epidermal keratinocyte (REK) Organotypic culture (ROC) is an epidermis model that is robust and inexpensive to develop and maintain, and it has in previous studies been shown to have permeability characteristics close to those of human skin. Here, we characterize the model further by structural comparison to native human and rat skin and by investigating functional characteristics of lipid packing, polarity, and permeability coefficients. We show that the ROC model has structural similarities to native human skin and observe human skin-like permeability coefficients for testosterone and mannitol. We develop a transwell device that allows live cell microscopy of the tissue at the air-liquid interface and establish transgenic cell lines expressing different fluorescently tagged proteins. This enables showing the migration of keratinocytes during the first days after seeding, finding that keratinocytes have a higher mean migration rate in the earlier days of development. Collectively, our results show that the ROC model is an inexpensive and robust epidermis model that works reproducibly across laboratories.
大鼠表皮角质形成细胞（REK）器官型培养（ROC）是一种表皮模型，其开发和维护成本低廉且稳健，并且在之前的研究中已经显示出与人体皮肤接近的通透性特征。在这里，我们通过与天然人皮肤和大鼠皮肤的结构比较以及研究脂质堆积、极性和渗透系数的功能特性来进一步描述该模型。我们表明，ROC 模型与人皮肤具有相似的结构，并观察到睾酮和甘露醇的人皮肤样渗透系数。我们开发了一种 Transwell 装置，允许在气液界面处对组织进行活细胞显微镜检查，并建立了表达不同荧光标记蛋白的转基因细胞系。这使得能够显示接种后最初几天角质形成细胞的迁移，发现角质形成细胞在发育的早期阶段具有更高的平均迁移率。总之，我们的结果表明，ROC 模型是一种廉价且稳健的表皮模型，在不同实验室中可重复使用。
