Dr Nafiz Körez Sincan State Hospital, Department of Otolaryngology Ankara, Turkey.
Hacettepe University Faculty of Medicine, Department of Otolaryngology Ankara, Turkey.
Auris Nasus Larynx. 2023 Jun;50(3):395-402. doi: 10.1016/j.anl.2022.08.003. Epub 2022 Aug 20.
To examine the protective effects of melatonin on laryngeal radiation damage.
31 rats were divided into 4 groups as follows: 1) the control (C) group (n=7), was only injected with intraperitoneal ethanol solution; 2) the melatonin (M) group (n=8), was injected intraperitoneal melatonin solution with 5 mg/kg; 3) the radiotherapy (RT) group (n=8) was given laryngeal radiation after intraperitoneal injection of ethanol solution; 4) the M + RT group (n=8), RT was given 30 minutes after 5 mg/kg dose of melatonin solution was injected. Drug and radiation applications were continued for 5 days. The weight changes of the rats were recorded. At the end of the study, inflammation, neutrophil migration and lymphoid aggregates, collagen distribution, laryngeal glandular structures and biochemical analysis of laryngeal tissues [malondialdehyde (MDA), total oxidant status (TOS) and total antioxidant status (TAS)] were determined.
In the M+RT group, the first day and the 30th weight differences were significantly increased when compared with the RT group (p=0.050). Inflammation, neutrophil migration, lymphoid aggregate, disorganized collagen distribution and loss of glandular tissues were found statistically more in the RT group than in the C group (p<0.05). MDA and TOS levels were in the M + RT group exhibited better values than they did in the RT group (p<0.05). TAS levels was markedly increased in the M + RT group than in the RT group (p<0.001).
Administration of melatonin to rats prior receiving laryngeal radiation, decreases the level of oxidative stress markers and increases the level of anti-oxidative markers.
探讨褪黑素对喉放射性损伤的保护作用。
将 31 只大鼠分为 4 组：1）对照组（C 组，n=7），仅注射腹腔乙醇溶液；2）褪黑素（M 组，n=8），注射 5mg/kg 剂量的腹腔褪黑素溶液；3）放射治疗（RT 组，n=8），给予腹腔乙醇溶液注射后行喉放射治疗；4）M+RT 组（n=8），给予 5mg/kg 剂量的褪黑素溶液 30 分钟后行 RT。药物和放射治疗持续 5 天。记录大鼠体重变化。研究结束时，评估大鼠炎症、中性粒细胞迁移和淋巴聚集、胶原分布、喉腺结构以及喉组织的生化分析[丙二醛（MDA）、总氧化剂状态（TOS）和总抗氧化状态（TAS）]。
与 RT 组相比，M+RT 组在第 1 天和第 30 天的体重差异显著增加（p=0.050）。与 C 组相比，RT 组大鼠炎症、中性粒细胞迁移、淋巴聚集、胶原分布紊乱和腺组织丧失更为明显（p<0.05）。与 RT 组相比，M+RT 组 MDA 和 TOS 水平更好（p<0.05）。与 RT 组相比，M+RT 组 TAS 水平显著升高（p<0.001）。
在接受喉放射治疗前给予大鼠褪黑素可降低氧化应激标志物水平并提高抗氧化标志物水平。
