Kushner L, Schulz H
Life Sci. 1987 Jul 27;41(4):485-90. doi: 10.1016/0024-3205(87)90225-6.
3-Mercaptopropanoyl-CoA and S-acetyl-3-mercaptopropanoyl-CoA, physiological metabolites of the known convulsant 3-mercaptopropanoic acid, were found to be inhibitors of purified pyruvate dehydrogenase complex from porcine and bovine heart. Under optimal conditions, 50% inhibition was obtained at 12.6 microM 3-mercaptopropanoyl-CoA or 5 microM S-acetyl-3-mercaptopropanoyl-CoA. The inhibition caused by S-acetyl-3-mercaptopropanoyl-CoA was irreversible. Maximal inhibition of the complex was observed when it was preincubated with the inhibitor under conditions which promote reduction of the endogenous lipoate.
