Amezian Dries, Mehlhorn Sonja, Vacher-Chicane Calypso, Nauen Ralf, Le Goff Gaëlle
Université Côte d'Azur, INRAE, CNRS, ISA, F-06903, Sophia Antipolis, France.
Bayer AG, Crop Science Division, R&D, Alfred Nobel-Strasse 50, 40789 Monheim, Germany.
Curr Res Insect Sci. 2022 Apr 18;2:100037. doi: 10.1016/j.cris.2022.100037. eCollection 2022.
(fall armyworm) is a highly destructive invasive pest that feeds on numerous crops including maize and rice. It has developed sophisticated mechanisms to detoxify xenobiotics such as secondary plant metabolites as well as manmade insecticides. The aim of the study was to explore the detoxification response to plant secondary metabolites and insecticides employing a Sf9 cell model exposed to indole 3-carbinol (I3C) and methoprene. The cell Inhibitory Concentration 50 (IC) for these molecules was determined and IC, IC and IC doses were used to monitor the induction profiles of detoxification genes. Cytochrome P450 monooxygenases (P450s) of the subfamily were the most inducible genes of the seven examined. Our results also showed the induction of the transcription factor Cap'n'collar isoform C (CncC). Transient transformation of Sf9 cells overexpressing CncC and its partner muscle aponeurosis fibromatosis () induces overexpression of and while CYP9As were not induced. Next, we determined the capacity of recombinantly expressed CYP9A30, CYP9A31 and CYP9A32 to interact with methoprene and I3C. Fluorescence-based biochemical assays revealed an interaction of methoprene with functionally expressed CYP9A30, CYP9A31 and CYP9A32 whereas almost no interaction was detected for I3C, suggesting the ability of CYP9As to metabolize methoprene. Our results showed that Sf9 cells could be a useful model to decipher detoxification pathways of .
草地贪夜蛾是一种极具破坏性的入侵害虫，以包括玉米和水稻在内的多种作物为食。它已形成了复杂的机制来解毒异生素，如植物次生代谢产物以及人造杀虫剂。本研究的目的是利用暴露于吲哚 - 3 - 甲醇（I3C）和烯虫酯的Sf9细胞模型，探索对植物次生代谢产物和杀虫剂的解毒反应。测定了这些分子的细胞抑制浓度50（IC），并使用IC、IC和IC剂量来监测解毒基因的诱导情况。在检测的七个基因中，亚家族的细胞色素P450单加氧酶（P450s）是最易诱导的基因。我们的结果还显示了转录因子Cap'n'collar异构体C（CncC）的诱导。过表达CncC及其伴侣肌腱膜纤维瘤病（）的Sf9细胞的瞬时转化诱导了和的过表达，而CYP9As未被诱导。接下来，我们测定了重组表达的CYP9A30、CYP9A31和CYP9A32与烯虫酯和I3C相互作用的能力。基于荧光的生化分析显示烯虫酯与功能表达的CYP9A30、CYP9A31和CYP9A32相互作用，而未检测到I3C有几乎任何相互作用，这表明CYP9As能够代谢烯虫酯。我们的结果表明，Sf9细胞可能是一个用于破译草地贪夜蛾解毒途径的有用模型。
