NTT Device Technology Laboratory, NTT Corporation, Atsugi 243-0198, Kanagawa, Japan.
Biosensors (Basel). 2022 Aug 8;12(8):615. doi: 10.3390/bios12080615.
We developed a novel measuring and data-processing method for performing electrochemical surface plasmon resonance (EC-SPR) on sensor surfaces for which detecting a specific SPR angle is difficult, such as a polymer having a non-uniform thickness with coloration. SPR measurements are used in medicine and basic research as an analytical method capable of molecular detection without labeling. However, SPR is not good for detecting small molecules with small refractive index changes. The proposed EC-SPR, which combines SPR measurements with an electrochemical reaction, makes it possible to measure small molecules without increasing the number of measurement steps. A drawback of EC-SPR is that it is difficult to detect a specific SPR angle on electron mediators, and it was found that it may not be possible to capture all the features produced. The novel method we describe here is different from the conventional one in which a specific SPR angle is obtained from an SPR curve; rather, it processes the SPR curve itself and can efficiently aggregate the feature displacements in the SPR curves that are dispersed through multiple angles. As an application, we used our method to detect small concentrations of HO (LOD 0.7 μM) and glutamate (LOD 5 μM).
我们开发了一种新的测量和数据处理方法，用于对传感器表面进行电化学表面等离子体共振（EC-SPR）测量，这种传感器表面的检测具有特定的 SPR 角度比较困难，例如具有变色的非均匀厚度的聚合物。SPR 测量在医学和基础研究中作为一种分析方法被广泛应用，它可以在不进行标记的情况下进行分子检测。然而，SPR 并不擅长检测具有小折射率变化的小分子。我们提出的将 SPR 测量与电化学反应相结合的 EC-SPR 方法，可以在不增加测量步骤的情况下测量小分子。EC-SPR 的一个缺点是，在电子介体上很难检测到特定的 SPR 角度，并且可能无法捕获所有产生的特征。我们在这里描述的新方法与传统方法不同，传统方法是从 SPR 曲线中获得特定的 SPR 角度，而是对 SPR 曲线本身进行处理，并且可以有效地聚集通过多个角度分散的 SPR 曲线上的特征位移。作为应用，我们使用我们的方法来检测低浓度的 HO（LOD 0.7 μM）和谷氨酸（LOD 5 μM）。
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