Altunbaş Canan, Aslan Ahmet, Kuşat Kevser, Sahiner Mehtap, Akgöl Sinan, Sahiner Nurettin
Department of Biochemistry, Faculty of Science, Ege University, Izmir 35100, Turkey.
Department of Leather Engineering, Faculty of Engineering, Ege University, Izmir 35100, Turkey.
Gels. 2022 Aug 12;8(8):501. doi: 10.3390/gels8080501.
The advantages of cryogels for enzyme immobilization applications include their mechanical and chemical robustness, ease of production, superior porosity, and low cost. Currently, many researchers are exploring porous material-based systems for enzyme immobilization that are more efficient and economically viable. Here, poly(2-Hydroxyethyl methacrylate-co-allyl glycidyl ether) (p(HEMA-co-AGE)) cryogel matrices were synthesized via the free radical cryopolymerization method to be employed as the support material. For the immobilization of the catalase enzyme onto the p(HEMA-co-AGE) cryogel matrix (catalase@p(HEMA-co-AGE), the best possible reaction conditions were determined by altering parameters such as pH, catalase initial concentration, and flow rate. The maximum catalase immobilization amount onto the p(HEMA-co-AGE) cryogel was found to be 48 mg/g cryogel. To determine the advantages of the cryogel matrix, e.g., the stability and reusability of the cryogel matrix, the adsorption-desorption cycles for the catalase enzyme were repeated five times using the same cryogel matrix. At the end of the reusability tests, it was found that the cryogel was very stable and maintained its adsorption capacity with the recovery ratio of 93.8 ± 1.2%. Therefore, the p(HEMA-co-AGE) cryogel matrix affords repeated useability, e.g., up to five times, without decreasing its catalase binding capacities significantly and has promising potential for many industrial applications. Cryogels offer clear distinctive advantages over common materials, e.g., micro/nano particles, hydrogels, films, and composites for these applications. At present, many researchers are working on the design of more effective and economically feasible, porous material-based systems for enzyme immobilization.
用于酶固定化应用的冷冻凝胶的优点包括其机械和化学稳定性、易于生产、优异的孔隙率和低成本。目前，许多研究人员正在探索基于多孔材料的酶固定化系统，这些系统更高效且经济可行。在此，通过自由基冷冻聚合法合成了聚（甲基丙烯酸2-羟乙酯-共-烯丙基缩水甘油醚）（p(HEMA-co-AGE)）冷冻凝胶基质，用作支撑材料。为了将过氧化氢酶固定在p(HEMA-co-AGE)冷冻凝胶基质上（过氧化氢酶@p(HEMA-co-AGE)），通过改变pH值、过氧化氢酶初始浓度和流速等参数来确定最佳反应条件。发现p(HEMA-co-AGE)冷冻凝胶上过氧化氢酶的最大固定量为48 mg/g冷冻凝胶。为了确定冷冻凝胶基质的优点，例如冷冻凝胶基质的稳定性和可重复使用性，使用相同的冷冻凝胶基质对过氧化氢酶进行了五次吸附-解吸循环。在可重复使用性测试结束时，发现冷冻凝胶非常稳定，其吸附容量得以保持，回收率为93.8±1.2%。因此，p(HEMA-co-AGE)冷冻凝胶基质具有重复使用性，例如可重复使用多达五次，而不会显著降低其过氧化氢酶结合能力，并且在许多工业应用中具有广阔的潜力。对于这些应用，冷冻凝胶相对于常见材料，例如微/纳米颗粒、水凝胶、薄膜和复合材料，具有明显的独特优势。目前，许多研究人员正在致力于设计更有效且经济可行的基于多孔材料的酶固定化系统。
