Zhou Qi, Wei Zihao, Xu Yanan, Xue Changhu
College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China.
Qingdao National Laboratory for Marine Science and Technology, Qingdao 266235, China.
Gels. 2022 Aug 19;8(8):517. doi: 10.3390/gels8080517.
This study aimed to explore the effects and mechanisms of differently shaped aggregates of ovotransferrin (OVT) particles on oleogel-based Pickering emulsions (OPEs). Medium-chain triglyceride oil-based oleogels were constructed using beeswax, and their gel-sol melting temperatures were investigated. Atomic force microscopy confirmed that both OVT fibrils and OVT spheres were successfully prepared, and the three-phase contact angle measurements indicated that fibrous and spherical aggregates of OVT particles possessed great potential to stabilize the OPEs. Afterward, the oil-in-water OPEs were fabricated using oleogel as the oil phase and OVT fibrils/spheres as the emulsifiers. The results revealed that OPEs stabilized with OVT fibrils (FIB-OPEs) presented a higher degree of emulsification, smaller droplet size, better physical stability and stronger apparent viscosity compared with OPEs stabilized with OVT spheres (SPH-OPEs). The freeze-thaw stability test showed that the FIB-OPEs remained stable after three freeze-thaw cycles, while the SPH-OPEs could barely withstand one freeze-thaw cycle. An in vitro digestion study suggested that OVT fibrils conferred distinctly higher lipolysis (46.0%) and bioaccessibility (62.8%) of curcumin to OPEs.
本研究旨在探究不同形状的卵转铁蛋白（OVT）颗粒聚集体对基于油凝胶的皮克林乳液（OPEs）的影响及其作用机制。使用蜂蜡构建了中链甘油三酯油基油凝胶，并对其凝胶 - 溶胶熔化温度进行了研究。原子力显微镜证实成功制备了OVT原纤维和OVT球体，三相接触角测量表明OVT颗粒的纤维状和球状聚集体具有稳定OPEs的巨大潜力。随后，以油凝胶为油相，以OVT原纤维/球体为乳化剂制备了水包油型OPEs。结果表明，与用OVT球体稳定的OPEs（SPH - OPEs）相比，用OVT原纤维稳定的OPEs（FIB - OPEs）具有更高的乳化程度、更小的液滴尺寸、更好的物理稳定性和更强的表观粘度。冻融稳定性测试表明，FIB - OPEs在三个冻融循环后仍保持稳定，而SPH - OPEs几乎无法承受一个冻融循环。体外消化研究表明，OVT原纤维赋予OPEs中姜黄素明显更高的脂解率（46.0%）和生物可及性（62.8%）。
