Dab Sandeep, Abdelhay Nancy, Figueredo Carlos Alberto, Ganatra Seema, Gibson Monica Prasad
School of Dentistry, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, AB T6G1C9, Canada.
Faculty of Dentistry, Alexandria University, Alexandria 5423012, Egypt.
Dent J (Basel). 2022 Aug 4;10(8):144. doi: 10.3390/dj10080144.
The SIBLING proteins are a family of non-collagenous proteins (NCPs) previously thought to be expressed only in dentin but have been demonstrated in other mineralized and non-mineralized tissues. They are believed to play vital roles in both osteogenesis and dentinogenesis. Since they are tightly regulated lifelong processes and involve a peak of mineralization, three different age groups were investigated. Fifteen wild-type (WT) mice were euthanized at ages 1, 3, and 6 months. Hematoxylin and eosin staining (H&E) was performed to localize various microscopic structures in the mice mandibles and tibias. The immunostaining pattern was compared using antibodies for dentin sialoprotein (DSP), dentin matrix protein 1 (DMP1), bone sialoprotein (BSP), and osteopontin (OPN). Immunostaining of DSP in tibia showed its most noticeable staining in the 3-month age group. DSP was expressed in alveolar bone, cellular cementum, and PDL. A similar expression of DMP1 was seen in the tibia and dentin. BSP was most noticeably detected in the tibia and acellular cementum. OPN was mainly expressed in the bone. A lower level of OPN was observed at all age groups in the teeth. The immunostaining intensity was the least detected for all proteins in the 6-month tibia sample. The expression patterns of the four SIBLING proteins showed variations in their staining intensity and temporospatial patterning concordant with skeletal and dental maturity. These findings suggest some role in this tightly regulated mineralization process.
SIBLING蛋白是一类非胶原蛋白（NCPs），以前认为仅在牙本质中表达，但已在其他矿化和非矿化组织中得到证实。它们被认为在骨生成和牙本质生成中都起着至关重要的作用。由于它们是受到严格调控的终身过程且涉及矿化高峰，因此对三个不同年龄组进行了研究。15只野生型（WT）小鼠在1、3和6个月龄时实施安乐死。进行苏木精和伊红染色（H&E）以定位小鼠下颌骨和胫骨中的各种微观结构。使用针对牙本质涎蛋白（DSP）、牙本质基质蛋白1（DMP1）、骨涎蛋白（BSP）和骨桥蛋白（OPN）的抗体比较免疫染色模式。胫骨中DSP的免疫染色显示其在3个月龄组中染色最为明显。DSP在牙槽骨、细胞性牙骨质和牙周膜中表达。在胫骨和牙本质中观察到DMP1的类似表达。BSP在胫骨和无细胞牙骨质中检测最为明显。OPN主要在骨中表达。在所有年龄组的牙齿中观察到较低水平的OPN。在6个月龄的胫骨样本中，所有蛋白质的免疫染色强度检测到最低。四种SIBLING蛋白的表达模式显示出它们的染色强度和时空模式变化，与骨骼和牙齿成熟度一致。这些发现表明在这个严格调控的矿化过程中具有一定作用。
