Bronckers A L, Farach-Carson M C, Van Waveren E, Butler W T
Department of Oral Cell Biology, Academic Center for Dentistry in Amsterdam (ACTA), Vrije Universiteit, The Netherlands.
J Bone Miner Res. 1994 Jun;9(6):833-41. doi: 10.1002/jbmr.5650090609.
Using immunohistochemical methods we studied the tissue localization of the extracellular matrix proteins osteopontin (OPN), osteocalcin (OC), and dentin sialoprotein (DSP) during the formation of acellular and cellular cementum in newly born rats. In the layer of acellular cementum of developing incisor and molar teeth we found a very strong staining for OPN but not for DSP or OC. Many cells immediately adjacent to acellular cementum and PDL cells were also positive for OPN but not for DSP or for OC. In contrast, cellular cementum in molar teeth stained strongly for OPN and OC but not for DSP. Consistent with these observations, the cells engaged in the formation of cellular cementum (cementoblasts and cementocytes) reacted strongly for OPN and OC but not for DSP. In advanced stages of dentinogenesis, both crown and root odontoblasts and dentin stained for OPN, OC, and DSP. Cells and matrices of surrounding alveolar bone stained for OPN and OC but not for DSP. We conclude that cementoblasts and cementocytes of cellular cementum produce OPN and OC but not DSP and thus express an osteoblast-like, not an odontoblast-like, phenotype. The cells responsible for the production of acellular cementum are likely cells of the PDL in close contact with the dental root surface. These fibroblast-like cells express OPN but not OC or DSP and accordingly express only a partial osteoblastic phenotype.
我们采用免疫组化方法，研究了新生大鼠无细胞牙骨质和细胞牙骨质形成过程中细胞外基质蛋白骨桥蛋白（OPN）、骨钙素（OC）和牙本质涎蛋白（DSP）的组织定位。在发育中的切牙和磨牙的无细胞牙骨质层，我们发现OPN染色非常强，而DSP和OC染色不强。许多紧邻无细胞牙骨质的细胞和牙周膜细胞OPN染色也呈阳性，但DSP和OC染色阴性。相反，磨牙的细胞牙骨质OPN和OC染色强，而DSP染色不强。与这些观察结果一致，参与细胞牙骨质形成的细胞（成牙骨质细胞和牙骨质细胞）OPN和OC染色强，而DSP染色阴性。在牙本质形成的晚期，冠部和根部的成牙本质细胞及牙本质OPN、OC和DSP染色均呈阳性。周围牙槽骨的细胞和基质OPN和OC染色阳性，但DSP染色阴性。我们得出结论，细胞牙骨质的成牙骨质细胞和牙骨质细胞产生OPN和OC，但不产生DSP，因此表现出成骨细胞样而非成牙本质细胞样表型。负责产生无细胞牙骨质的细胞可能是与牙根表面紧密接触的牙周膜细胞。这些成纤维细胞样细胞表达OPN，但不表达OC或DSP，因此仅表现出部分成骨细胞表型。
