Zhu Hang, Liu Zheming, Ma Haihao, Zheng Wei, Liu Jia, Zhou Yong, Man Yilong, Zhou Xiaoao, Zeng Aiping
College of Plant Protection, Hunan Agricultural University, Changsha 410128, China.
Institute of Agricultural Biotechnology, Hunan Academy of Agricultural Sciences, Changsha 410125, China.
Insects. 2022 Aug 16;13(8):735. doi: 10.3390/insects13080735.
The diamondback moth () is one of the most destructive lepidopteran pests of cruciferous vegetables, and insights into regulation of its physiological processes contribute towards the development of new pesticides against it. Thus, we investigated the regulatory functions of its β-adrenergic-like octopamine receptor (PxOctβ3). The open reading frame (ORF) of was phylogenetically analyzed, and the levels of expression of the receptor mRNA were determined. This ORF was also cloned and expressed in HEK-293 cells. A series of octopamine receptor agonists and antagonists were tested against PxOctβ3. We showed that the receptor is a member of the Octβ3 protein family, and an analysis using quantitative PCR showed that it was expressed at all developmental stages of . Octopamine activated PxOctβ3, resulting in increased levels of intracellular cAMP. Furthermore, the agonists naphazoline, clonidine, 2-phenethylamine, and amitraz activated the PxOctβ3 receptor, and naphazoline was the most effective. Only metoclopramide and mianserin had significant antagonistic effects on PxOctβ3, whereas yohimbine, phentolamine, and chlorpromazine lacked obvious antagonistic effects. The injection of double-stranded RNA in an RNA interference assay indicated that PxOctβ3 regulates development in . This study demonstrated the pharmacological properties and functions of PxOctβ3 in , thus, providing a theoretical basis for the design of pesticides that target octopamine receptors.
小菜蛾是十字花科蔬菜最具破坏性的鳞翅目害虫之一，深入了解其生理过程的调控有助于开发新型杀虫剂来防治该害虫。因此，我们研究了其β-肾上腺素能样章鱼胺受体（PxOctβ3）的调控功能。对PxOctβ3的开放阅读框（ORF）进行了系统发育分析，并测定了该受体mRNA的表达水平。还将此ORF克隆并在HEK-293细胞中进行表达。对一系列章鱼胺受体激动剂和拮抗剂针对PxOctβ3进行了测试。我们发现该受体是Octβ3蛋白家族的成员，定量PCR分析表明其在小菜蛾各个发育阶段均有表达。章鱼胺激活PxOctβ3，导致细胞内cAMP水平升高。此外，激动剂萘甲唑啉、可乐定、2-苯乙胺和阿米特拉嗪激活了PxOctβ3受体，其中萘甲唑啉最为有效。只有甲氧氯普胺和米安色林对PxOctβ3有显著拮抗作用，而育亨宾、酚妥拉明和氯丙嗪缺乏明显的拮抗作用。RNA干扰试验中注射双链RNA表明PxOctβ3调控小菜蛾的发育。本研究证明了PxOctβ3在小菜蛾中的药理学特性和功能，从而为设计靶向章鱼胺受体的杀虫剂提供了理论依据。
