Banerjee Priyanka, Rodning Soren P, Diniz Wellison J S, Dyce Paul W
Department of Animal Sciences, Auburn University, Auburn, AL 36849, USA.
Metabolites. 2022 Jul 29;12(8):708. doi: 10.3390/metabo12080708.
Reproductive failure remains a significant challenge to the beef industry. The omics technologies have provided opportunities to improve reproductive efficiency. We used a multistaged analysis from blood profiles to integrate metabolome (plasma) and transcriptome (peripheral white blood cells) in beef heifers. We used untargeted metabolomics and RNA-Seq paired data from six AI-pregnant (AI-P) and six nonpregnant (NP) Angus-Simmental crossbred heifers at artificial insemination (AI). Based on network co-expression analysis, we identified 17 and 37 hub genes in the AI-P and NP groups, respectively. Further, we identified , , , , and as more connected in the NP heifers' network. The NP gene network showed a connectivity gain due to the rewiring of major regulators. The metabolomic analysis identified 18 and 15 hub metabolites in the AI-P and NP networks. Tryptophan and allantoic acid exhibited a connectivity gain in the NP and AI-P networks, respectively. The gene-metabolite integration identified tocopherol-a as positively correlated with ENSBTAG00000009943 in the AI-P group. Conversely, tocopherol-a was negatively correlated in the NP group with , , and . In the NP group, α-ketoglutarate- and putrescine- were positively correlated, whereas a-ketoglutarate- and tryptophan- were negatively correlated. These multiple interactions identified novel targets and pathways underlying fertility in bovines.
繁殖失败仍然是牛肉行业面临的重大挑战。组学技术为提高繁殖效率提供了机会。我们对肉牛小母牛进行了从血液分析到整合代谢组（血浆）和转录组（外周血白细胞）的多阶段分析。我们使用了来自六头人工授精怀孕（AI-P）和六头未怀孕（NP）的安格斯-西门塔尔杂交小母牛在人工授精（AI）时的非靶向代谢组学和RNA测序配对数据。基于网络共表达分析，我们在AI-P组和NP组中分别鉴定出17个和37个枢纽基因。此外，我们发现在NP小母牛的网络中，[具体基因1]、[具体基因2]、[具体基因3]、[具体基因4]和[具体基因5]的连接性更强。NP基因网络由于主要调节因子的重新连接而显示出连接性增加。代谢组学分析在AI-P和NP网络中分别鉴定出18个和15个枢纽代谢物。色氨酸和尿囊酸分别在NP和AI-P网络中显示出连接性增加。基因-代谢物整合表明，在AI-P组中生育三烯酚-α与ENSBTAG00000009943呈正相关。相反，在NP组中生育三烯酚-α与[具体基因1]、[具体基因2]和[具体基因3]呈负相关。在NP组中，α-酮戊二酸和腐胺呈正相关，而α-酮戊二酸和色氨酸呈负相关。这些多重相互作用确定了牛生育力潜在的新靶点和途径。
