Wientroub S, Fisher L W, Reddi A H, Termine J D
Mol Cell Biochem. 1987 Apr;74(2):157-62. doi: 10.1007/BF00224953.
Rats were raised in the absence of vitamin D in utero and throughout post-fetal life and neither 1,25-dihydroxyvitamin D3 nor related metabolites were detected in serums. No changes were observed in the relative amount of extractable noncollagenous bone proteins (NCP) in rachitic compared to vitamin-D-repleted animals. As expected, the relative levels of the mineral-bound, serum-derived albumin and alpha 2HS glycoprotein were unaffected in bones of rachitic animals. Interestingly, the vitamin D deficiency also did not have dramatic effects on several bone cell-derived noncollagenous proteins including: bone proteoglycans I & II, bone sialoprotein II osteonectin, and osteocalcin. In contrast to the proteoglycans, the bone sialoprotein II and osteonectin were found in the nonmineral compartment of the rachitic animals, presumably bound to the wide osteoid seam.
大鼠在子宫内及整个胎儿期后均在缺乏维生素D的环境中饲养，血清中未检测到1,25-二羟基维生素D3及其相关代谢物。与补充维生素D的动物相比，佝偻病动物中可提取的非胶原蛋白骨蛋白（NCP）的相对含量未观察到变化。正如预期的那样，矿物质结合的、血清来源的白蛋白和α2HS糖蛋白的相对水平在佝偻病动物的骨骼中未受影响。有趣的是，维生素D缺乏对几种骨细胞衍生的非胶原蛋白也没有显著影响，这些蛋白包括：骨蛋白聚糖I和II、骨唾液酸蛋白II、骨连接素和骨钙素。与蛋白聚糖不同，骨唾液酸蛋白II和骨连接素存在于佝偻病动物的非矿物质部分，可能与宽阔的类骨质缝结合。
