Bolwell Charlotte, Gee Erica, Adams Brooke, Collins-Emerson Julie, Scarfe Katherine, Nisa Shahista, Gordon Emma, Rogers Chris, Benschop Jackie
School of Veterinary Science, Massey University, Palmerston North 4442, New Zealand.
IDEXX Laboratories (NZ) ULC, School of Veterinary Science Complex, Massey University, Palmerston North 4442, New Zealand.
Vet Sci. 2022 Aug 12;9(8):426. doi: 10.3390/vetsci9080426.
The objectives of this study were to determine if horses located near an outbreak of leptospirosis in alpacas had titres indicative of a previous or current infection and, if so, to determine the magnitude in change of titres over time. Further, the objective was to determine if horses with high titre results were shedding in their urine. Blood samples were collected from twelve horses located on or next to the farm with the outbreak in alpacas, on day zero and at four subsequent time points (two, four, six and nine weeks). The microscopic agglutination test was used to test sera for five serovars endemic in New Zealand: Ballum, Copenhageni, Hardjo, Pomona and Tarassovi. A reciprocal MAT titre cut-off of ≥1:100 was used to determine positive horses. Seven out of twelve horses (58%) were positive to at least one serovar during one of the time points. Two horses recorded titres of ≥1600, one for both Pomona and Copenhageni and the other for Hardjo, and these two horses were both PCR positive for in their urine samples. For five out of seven horses, the titres either remained the same or changed by one dilution across the sampling time points. The study confirmed endemic exposure to five endemic serovars in New Zealand in a group of horses located near a confirmed leptospirosis outbreak in alpacas.
本研究的目的是确定位于羊驼钩端螺旋体病暴发附近的马匹是否具有表明既往或当前感染的滴度，如果是，则确定滴度随时间变化的幅度。此外，目的是确定滴度高的马匹是否通过尿液排毒。在零日以及随后的四个时间点（两周、四周、六周和九周），从位于发生羊驼疫情的农场或其附近的12匹马采集血样。采用显微镜凝集试验检测新西兰流行的5种血清型：巴伦型、哥本哈根型、哈焦型、波莫纳型和塔拉索维型的血清。使用≥1:100的倒数MAT滴度截断值来确定阳性马匹。12匹马中有7匹（58%）在其中一个时间点对至少一种血清型呈阳性。两匹马的滴度≥1600，一匹对波莫纳型和哥本哈根型均呈阳性，另一匹对哈焦型呈阳性，这两匹马的尿液样本PCR检测均为阳性。在7匹马中的5匹中，滴度在整个采样时间点保持不变或仅改变一个稀释度。该研究证实，在一群位于确诊羊驼钩端螺旋体病暴发附近的马匹中，存在新西兰5种流行血清型的地方性暴露。
