Department of Biomedical Analysis, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, Tokyo, 192-0392, Japan.
Department of Biomedical Analysis, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, Tokyo, 192-0392, Japan.
Talanta. 2023 Jan 15;252:123827. doi: 10.1016/j.talanta.2022.123827. Epub 2022 Aug 14.
We developed a colorimetric analytical method for favipiravir (FPV), a promising treatment for COVID-19. FPV forms yellow complexes with ferrihydrite (Fh) by a ligand substitution reaction with the iron (III) hydroxyl surface groups in Fh. Fh-coated microbeads were packed into a capillary tube with an inner diameter of 1 mm. FPV-spiked serum after pretreatment was drawn into the capillary packed with Fh-coated microbeads. The intensities of the yellow-color complexes on the microbeads were transformed into red-green-blue (RGB) pixels using Image-J software 1.8, and the difference between green and blue was used as the analytical signal. The signal increased with increasing FPV concentration. The proposed method showed good linearity (R = 0.9907) over a concentration range of 25-200 μg/mL. The RSD (n = 3) and the LOD (3σ) values were estimated to be 2.8-15.4% and 16.91 μg/mL, respectively. The proposed method enables us to quantify FPV rapidly and easily without the need for expensive equipment.
我们开发了一种用于法匹拉韦（FPV）的比色分析方法，FPV 是治疗 COVID-19 的一种有前途的药物。FPV 通过与 Fh 中氧化铁（III）羟基表面基团的配体取代反应，与 Fh 形成黄色配合物。Fh 涂覆的微球被填充到内径为 1mm 的毛细管中。经过预处理后，含有 FPV 的血清被吸入填充有 Fh 涂覆的微球的毛细管中。使用 Image-J 软件 1.8 将微球上黄色配合物的强度转换为红-绿-蓝（RGB）像素，并且将绿色和蓝色之间的差异用作分析信号。随着 FPV 浓度的增加，信号增加。该方法在 25-200μg/mL 的浓度范围内表现出良好的线性（R=0.9907）。估计相对标准偏差（n=3）和检出限（3σ）值分别为 2.8-15.4%和 16.91μg/mL。该方法无需昂贵的设备即可快速、轻松地定量 FPV。
