Shi Xian, Xu Xidan, Shi Nian, Chen Yongjun, Fu Manni
Department of Dermatology, Huangshi Central Hospital, Huangshi, 435000, Hubei, China.
Department of Dermatology, Huangshi Central Hospital, Huangshi, 435000, Hubei, China.
Biochem Biophys Res Commun. 2022 Oct 30;627:60-67. doi: 10.1016/j.bbrc.2022.08.014. Epub 2022 Aug 9.
Melanoma, the type of skin cancer considered as most malignant, and known to be linked with a high incidence as well as high mortality rate. Although the dysregulation of ASF1B and miR-767-3p expression is involved in the progression of various cancers, their biological function in melanoma remains unclear.
Real-time qPCR was the primary source for determining the levels of ASF1B and miR-767-3p in melanoma. For the validation of association among miR-767-3p and ASF1B, luciferase activity assay was used. Quantification of cell apoptosis, proliferation, migration and viability in melanoma cells were carried out by flow cytometry, BrdU, transwell assays, and CCK-8, respectively. Further evaluation of tumor growth was achieved by xenograft in vivo.
Results showed an increased expression of ASF1B while declined expression of miR-767-3p in melanoma. ASF1B knockdown repressed cell migration, viability, proliferation, and tumor growth whereas boosted apoptosis in A375 as well as in A875 melanoma cells. Moreover, miR-767-3p attenuated the migration and proliferation of melanoma cells and encouraged cell apoptosis by reducing ASF1B levels.
In this study, miR-767-3p was shown to inhibit ASF1B which will attenuate melanoma tumorigenesis, and by this it can be a potential new effective biomarker for the treatment of melanoma.
黑色素瘤是最恶性的皮肤癌类型，其发病率和死亡率都很高。虽然ASF1B和miR-767-3p表达失调与多种癌症的进展有关，但其在黑色素瘤中的生物学功能仍不清楚。
实时定量PCR是测定黑色素瘤中ASF1B和miR-767-3p水平的主要方法。为验证miR-767-3p与ASF1B之间的关联，采用荧光素酶活性测定法。分别通过流式细胞术、BrdU、Transwell实验和CCK-8对黑色素瘤细胞的凋亡、增殖、迁移和活力进行定量分析。通过体内异种移植进一步评估肿瘤生长情况。
结果显示，黑色素瘤中ASF1B表达增加，而miR-767-3p表达下降。敲低ASF1B可抑制A375和A875黑色素瘤细胞的迁移、活力、增殖以及肿瘤生长，同时促进细胞凋亡。此外，miR-767-3p可通过降低ASF1B水平来减弱黑色素瘤细胞的迁移和增殖，并促进细胞凋亡。
在本研究中，miR-767-3p被证明可抑制ASF1B，从而减弱黑色素瘤的肿瘤发生，因此它可能是一种潜在的治疗黑色素瘤的新型有效生物标志物。
