State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210023, China.
State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210023, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2022 Dec 15;283:121635. doi: 10.1016/j.saa.2022.121635. Epub 2022 Jul 29.
Cysteine (Cys), one of the biological thiols, which plays critical roles in biological system regulating the balance of redox homeostasis. In order to monitor the level of Cys in the living cells and organisms, a chromogenic fluorescence probe Rhocl-Cys based on Rhodamine chloride exhibiting the preferable performance of fluorescence turn-on response reacting with Cys was presented. Rhocl-Cys responded rapidly to Cys within 20 min, and had stable fluorescence intensity within pH 6.0-10.0, high selectivity towards Cys and the anti-inference capability with a low detection limit of 0.80 μM. In particular, Rhocl-Cys could qualitatively and quantitatively monitor the level of endogenous and exogenous Cys in living cells and successfully apply to zebrafish detecting Cys. Therefore, these results might further provide the basis exploring the role of Cys in biological system and facilitate as clinical diagnostic molecular tools.
半胱氨酸（Cys）是一种生物硫醇，在调节氧化还原平衡的生物系统中发挥着关键作用。为了监测活细胞和生物体内 Cys 的水平，本文设计了一种基于罗丹明氯化物的比色荧光探针 Rhocl-Cys，它对 Cys 具有较好的荧光开响应性能。Rhocl-Cys 与 Cys 在 20 min 内快速响应，在 pH 6.0-10.0 范围内具有稳定的荧光强度，对 Cys 具有高选择性和抗干扰能力，检测限低至 0.80 μM。特别地，Rhocl-Cys 可以定性和定量监测活细胞内内源性和外源性 Cys 的水平，并成功应用于斑马鱼检测 Cys。因此，这些结果可能进一步为探索 Cys 在生物系统中的作用提供依据，并有助于开发临床诊断的分子工具。
