Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin Key Laboratory of Industrial Microbiology, College of Biotechnology, Tianjin University of Science & Technology, Tianjin 300457, China.
Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin Key Laboratory of Industrial Microbiology, College of Biotechnology, Tianjin University of Science & Technology, Tianjin 300457, China.
J Microbiol Methods. 2022 Sep;200:106559. doi: 10.1016/j.mimet.2022.106559. Epub 2022 Aug 22.
Strong-flavor Baijiu (SFB) is produced in complex fermentation in pits under ground. Clostridium producing hexanoic acid plays a key role in the flavor formation of SFB. The screening and culture for Clostridium are very difficult because of its strict anaerobic characteristics. In this study, electric field assisted screening (EFAS) was used to screen Clostridium from pit mud, and electric culture (EC) was used to cultivate Clostridium under non-anaerobic conditions. A strain with a high yield of hexanoic acid was screened and named as Clostridium sp. EFAS6. Under non-anaerobic conditions, it grew rapidly only near the cathode end in the EFAS device because of the low oxidation-reduction potential of that electrode. In the experiment of high-density culture in the EC device, the cell concentration reached 10-10. After energy consumption was calculated, the optimal loading voltage was found to be 10 V. In the application, the broth of Clostridium sp. EFAS6 increased the content of ethyl hexanoic in SFB. Under non-anaerobic conditions, the anaerobe was screened by EFAS and cultivated in high density by EC. The EFAS and EC could also be used for the screening and culture of other anaerobes under non-anaerobic conditions.
浓香型白酒（SFB）采用地下窖池进行复杂发酵生产。产生己酸的梭菌在 SFB 风味形成中起着关键作用。由于其严格的厌氧特性，梭菌的筛选和培养非常困难。在本研究中，电场辅助筛选（EFAS）用于从窖泥中筛选梭菌，并且在非厌氧条件下使用电动培养（EC）来培养梭菌。筛选出了一种己酸产量较高的菌株，并将其命名为梭菌 EFAS6。在非厌氧条件下，由于该电极的氧化还原电位较低，EFAS 装置中只有在阴极端附近才能快速生长。在 EC 装置中的高密度培养实验中，细胞浓度达到 10-10。在计算了能量消耗之后，发现最佳加载电压为 10 V。在应用中，梭菌 EFAS6 的发酵液增加了 SFB 中己酸乙酯的含量。在非厌氧条件下，通过 EFAS 筛选厌氧菌，并通过 EC 进行高密度培养。EFAS 和 EC 也可用于在非厌氧条件下筛选和培养其他厌氧菌。
