Luo Y M, Wang R, Bai Y N, Song N, Li M T, Zeng X F, Hu C J
Department of Rheumatology and Clinical Immunology, Peking Union Medical College Hospital, Peking Union Medical College,Chinese Academy of Medical Sciences, National Clinical Research Center for Dermatologic and Immunologic Diseases, Ministry of Science & Technology,State Key Laboratory of Complex Severe and Rare Diseases, Key Laboratory of Rheumatology and Clinical Immunology, Ministry of Education, Beijing 100730, China.
Zhonghua Nei Ke Za Zhi. 2022 Sep 1;61(9):1044-1048. doi: 10.3760/cma.j.cn112138-20210901-00607.
To investigate the distribution and clinical significance of nuclear dense fine speckled (DFS) pattern in various diseases. A total of 95 289 patients who received DFS tests at Peking Union Medical College Hospital from January 2019 to December 2020 were included in this study. The results of indirect immunofluorescence assay (IIF) for detection of antinuclear antibody (ANA) were evaluated. The positive rates of ANA and DFS were 39.60% (37 733/95 289) and 1.19% (1 139/95 289) respectively. The positive rate of DFS in ANA-positive patients was 3.02% (1 139/37 733). DFS and ANA positivity were significantly different among different age groups rather than gender. The positivity rate of DFS reached the peak (55.57%, 633/1 139) in young patients between 21-40 years, while positive ANA with negative DFS was mainly observed in patients between 41-60 years (37.26%, 13 636/36 594). Additionally, single ANA-positivity were mainly detected in rheumatology department (59.23%, 18 402/31 066), whereas positive DFS was more common in obstetrics and gynecology department (3.08%, 49/1 593). There were 82.88% (944/1 139) patients with positive DFS diagnosed with non-autoimmune disease (non-AID), and 19.49%(222/1 139) with dermatosis. Positive DFS with higher titer (≥1∶320) was detected more frequently in autoimmune disease (AID) patients (5.13%, 10/195) than in non-AID patients (1.69%, 16/944) (<0.05). The DFS pattern is rare in ANA positive patients, which is mainly observed in women between 21-49 years. High titer of DFS is prevalent in AID patients, but positive DFS is detected more in non-AID patients, especially those with dermatosis.
探讨核致密细颗粒状（DFS）模式在各种疾病中的分布及临床意义。本研究纳入了2019年1月至2020年12月在北京协和医院接受DFS检测的95289例患者。评估了间接免疫荧光法（IIF）检测抗核抗体（ANA）的结果。ANA和DFS的阳性率分别为39.60%（37733/95289）和1.19%（1139/95289）。ANA阳性患者中DFS的阳性率为3.02%（1139/37733）。DFS和ANA阳性在不同年龄组之间存在显著差异，而在性别方面无显著差异。DFS阳性率在21 - 40岁的年轻患者中达到峰值（55.57%，633/1139），而ANA阳性但DFS阴性主要见于41 - 60岁的患者（37.26%，13636/36594）。此外，单纯ANA阳性主要在风湿免疫科检测到（59.23%，18402/31066），而DFS阳性在妇产科更为常见（3.08%，49/1593）。DFS阳性患者中有82.88%（944/1139）被诊断为非自身免疫性疾病（非AID），19.49%（222/1139）患有皮肤病。自身免疫性疾病（AID）患者中检测到更高滴度（≥1∶320）的DFS阳性比非AID患者更频繁（5.13%，10/195比1.
