一种在静态条件下开发念珠菌生物膜的简单 96 孔板方法。
A Simple 96-Well Plate-Based Method for Development of Candida Biofilms Under Static Conditions.
机构信息
David Geffen School of Medicine, University of California (UCLA), Los Angeles, CA, USA.
Division of Infectious Disease, The Lundquist Institute for Biomedical Innovation at Harbor-UCLA Medical Center, Torrance, CA, USA.
出版信息
Methods Mol Biol. 2022;2542:225-231. doi: 10.1007/978-1-0716-2549-1_16.
We describe a rapid and simple in vitro method for development of Candida biofilms under static growth conditions. This 96-well microtiter-based method measures metabolic activity of sessile cells and can also be easily adapted for antifungal susceptibility testing. The entire procedure takes 2-3 days to complete, reliably quantifies biofilms, and provides reproducible results that are imperative toward the standardization of antifungal susceptibility testing of biofilms.
我们描述了一种在静态生长条件下快速简单地培养念珠菌生物膜的体外方法。这种基于 96 孔微量滴定板的方法可测量固定细胞的代谢活性，并且易于适应抗真菌药敏试验。整个过程需要 2-3 天完成，可可靠地定量生物膜，并提供可重复的结果，这对于生物膜抗真菌药敏试验的标准化至关重要。
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