Andreani Giulia, Dalmonte Thomas, Guerrini Alessandro, Lupini Caterina, Fabbri Micaela, Ferlizza Enea, Isani Gloria
Department of Veterinary Medical Sciences, University of Bologna, Via Tolara di Sopra 50, Ozzano dell'Emilia, 40064 Bologna, Italy.
Interdepartmental Centre for Agri-Food Industrial Research (CIRI Agrifood), University of Bologna, Cesena, Piazza G. Goidanich 60, 47521 Cesena, Italy.
Animals (Basel). 2022 Aug 9;12(16):2014. doi: 10.3390/ani12162014.
Extracts from () and () are used as supplements in poultry feed. The aims of this research were to study the possible effects of dietary supplementation with and extracts on serum and albumen proteins, zinc and iron, and yolk cholesterol content in Leghorn hens during the critical phase of the onset of laying. A total of 120 pullets, 17 weeks of age, were assigned to two groups (control (C) and treated (T), n = 60 each). The T group received a supplement containing (5%) and (5%) for 12 weeks. The study lasted 19 weeks. Serum proteins were fractionated using agarose gel electrophoresis (AGE) and SDS-polyacrylamide gel electrophoresis (SDS-PAGE). Trace elements were determined in serum using atomic absorption spectrometry, and yolk cholesterol was determined using a colorimetric test. No significant differences were observed between control and supplemented hens for the analyzed biochemical indices. Moreover, the supplementation with phytoextracts did not negatively affect the physiological variations in serum proteins; therefore, it can be safely used as a treatment to prevent inflammatory states at onset and during the early laying phase.
（）和（）的提取物被用作家禽饲料的补充剂。本研究的目的是研究在来航鸡产蛋开始的关键阶段，日粮中添加（）和（）提取物对血清和蛋清蛋白、锌和铁以及蛋黄胆固醇含量的可能影响。总共120只17周龄的小母鸡被分为两组（对照组（C）和处理组（T），每组n = 60）。处理组接受含有（5%）和（5%）的补充剂，持续12周。研究持续了19周。使用琼脂糖凝胶电泳（AGE）和SDS-聚丙烯酰胺凝胶电泳（SDS-PAGE）对血清蛋白进行分离。使用原子吸收光谱法测定血清中的微量元素，使用比色法测定蛋黄胆固醇。在分析的生化指标方面，对照组和补充组母鸡之间未观察到显著差异。此外，植物提取物的补充对血清蛋白的生理变化没有负面影响；因此，它可以安全地用作预防产蛋开始时和早期产蛋阶段炎症状态的治疗方法。
