De Felice Elena, Gatta Claudia, Giaquinto Daniela, Fioretto Federica, Maruccio Lucianna, d'Angelo Danila, Scocco Paola, de Girolamo Paolo, D'Angelo Livia
School of Biosciences and Veterinary Medicine, University of Camerino, Via Gentile III da Varano, 62032 Camerino, Italy.
Department of Veterinary Medicine and Animal Production, University of Naples Federico II, Via F. Delpino 1, 80137 Naples, Italy.
Animals (Basel). 2022 Aug 22;12(16):2148. doi: 10.3390/ani12162148.
First identified as an anorexigenic peptide, in the last decades, several studies have suggested that Nesfatin-1 (Nesf-1) is a pleiotropic hormone implicated in numerous regulatory processes in peripheral organs and tissues. In vertebrates, Nesf-1 is indeed expressed in the central nervous system and peripheral organs. In this study, we characterized the pattern of Nesf-1 distribution within the digestive tract of the common bottlenose dolphin (), composed of three gastric chambers and an intestine without a clear subdivision in the small and large intestine, also lacking a caecum. Our results indicated that Nesf-1 is widely distributed in cells of the mucosal epithelium of the gastric chambers. Most of the immunoreactivity was observed in the second chamber, compared to the first and third chambers. Immunopositivity was also found in nerve fibers and neurons, scattered or/and clustered in ganglion structures along all the examined gastrointestinal tracts. These observations add new data on the highly conserved role of Nesf-1 in the mammalian digestive system.
最初被鉴定为一种厌食肽，在过去几十年中，多项研究表明，内脂素-1（Nesf-1）是一种多效性激素，参与外周器官和组织中的众多调节过程。在脊椎动物中，Nesf-1确实在中枢神经系统和外周器官中表达。在本研究中，我们描述了宽吻海豚消化道内Nesf-1的分布模式，该消化道由三个胃腔和一段没有明显小肠和大肠细分且没有盲肠的肠道组成。我们的结果表明，Nesf-1广泛分布于胃腔黏膜上皮细胞中。与第一和第三胃腔相比，在第二胃腔中观察到的免疫反应性最强。在所有被检查的胃肠道中，沿着神经节结构分散或/和聚集的神经纤维和神经元中也发现了免疫阳性。这些观察结果为Nesf-1在哺乳动物消化系统中的高度保守作用增添了新的数据。
