Varricchio Ettore, Russolillo Maria Grazia, Russo Finizia, Lombardi Vittoria, Paolucci Marina, Maruccio Lucianna
Department of Sciences and Technologies, University of Sannio, Via Port'Arsa 11, 82100 Benevento, Italy.
Department of Sciences and Technologies, University of Sannio, Via Port'Arsa 11, 82100 Benevento, Italy; Centro Studi" Province of Benevento, University of Sannio, Contrada Piano Cappelle, 82100 Benevento, Italy.
Acta Histochem. 2014 May;116(4):583-7. doi: 10.1016/j.acthis.2013.11.006. Epub 2013 Dec 20.
In this study, we report nesfatin-1 immunoreactivity in the gastrointestinal tract of Casertana breed pig. The newly discovered anorexigenic peptide nesfatin-1 has been shown to possess physiological relevance in regulating food intake and energy homeostasis at a central level, although evidence has been accumulating that it may also play important functions at a more local gastroenteric level. Nesfatin-1 immunoreactive endocrine cells have been detected in the gastric fundus and ileocecal valve. Nesfatin-1 immunopositive neurons and nerve fibers have been observed mainly in the enteric plexuses. Western blot analysis confirmed the immunohistochemical observations, showing immunoreactive bands in all analyzed gastrointestinal tracts with the exception of the rectum. Nesfatin-1 immunodetection in the swine digestive system reinforces the importance of the role played by nesfatin-1 at the gastrointestinal level and sustains the necessity to study the role of this peptide in the regulation of food intake in farm species for which weight gain is essential for optimizing production.
在本研究中，我们报道了卡塞塔纳猪胃肠道中奈斯帕汀-1的免疫反应性。新发现的厌食肽奈斯帕汀-1已被证明在中枢水平调节食物摄入和能量平衡方面具有生理相关性，尽管越来越多的证据表明它在更局部的胃肠水平也可能发挥重要作用。在胃底和回盲瓣中检测到了奈斯帕汀-1免疫反应性内分泌细胞。主要在肠神经丛中观察到了奈斯帕汀-1免疫阳性神经元和神经纤维。蛋白质印迹分析证实了免疫组织化学观察结果，除直肠外，在所有分析的胃肠道中均显示出免疫反应条带。猪消化系统中的奈斯帕汀-1免疫检测强化了奈斯帕汀-1在胃肠道水平所起作用的重要性，并支持研究该肽在体重增加对优化生产至关重要的养殖物种食物摄入调节中的作用的必要性。
